CytoMatrix for a reliable and simple characterization of lung cancer stem cells from malignant pleural effusions.
Aged
Biomarkers, Tumor
/ metabolism
Carcinoma, Non-Small-Cell Lung
/ metabolism
Cell Culture Techniques
/ methods
Cell Nucleus
/ metabolism
Cytodiagnosis
/ methods
Cytoplasm
/ metabolism
Female
Humans
Lung
/ metabolism
Lung Neoplasms
/ metabolism
Male
Middle Aged
Neoplastic Stem Cells
/ metabolism
Pleural Effusion, Malignant
/ metabolism
Spheroids, Cellular
/ metabolism
Stearoyl-CoA Desaturase
/ metabolism
Transcription Factors
/ metabolism
Tumor Cells, Cultured
CytoMatrix
MPEs
SCD1
cancer stem cells
lung cancer
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
11
03
2019
accepted:
17
07
2019
pubmed:
10
8
2019
medline:
16
12
2020
entrez:
10
8
2019
Statut:
ppublish
Résumé
Cancer stem cells (CSCs) are a subpopulation with the properties of extensive self-renewal, capability to generate differentiated cancer cells and resistance to therapies. We have previously shown that malignant pleural effusions (MPEs) from patients with non-small-cell lung cancer (NSCLC) represent a valuable source of cancer cells that can be grown as three-dimensional (3D) spheroids enriched for stem-like features, which depend on the activation of the Yes-associated protein-transcriptional coactivator with PDZ-binding motif (YAP-TAZ)/Wnt-βcatenin/stearoyl-CoA desaturase 1 (SCD1) axis. Here, we describe a novel support, called CytoMatrix, for the characterization of limited amounts of cancer cells isolated from MPEs of patients with NSCLC. Our results show that this synthetic matrix allows an easy and fast characterization of several epithelial cellular markers. The use of CytoMatrix to study CSCs subpopulation confirms that SCD1 protein expression is enhanced in 3D spheroids when compared with 2D adherent cell cultures. YAP/TAZ nuclear-cytoplasmic distribution analysed by CytoMatrix in 3D spheroids is highly heterogeneous and faithfully reproduces what is observed in tumour biopsies. Our results confirm and extend the robustness of our workflow for the isolation and phenotypic characterization of primary cancer cells derived from the lung MPEs and underscore the role of SCD1.
Identifiants
pubmed: 31397494
doi: 10.1002/jcp.29121
pmc: PMC6916247
doi:
Substances chimiques
Biomarkers, Tumor
0
Transcription Factors
0
Stearoyl-CoA Desaturase
EC 1.14.19.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1877-1887Informations de copyright
© 2019 The Authors. Journal of Cellular Physiology published by Wiley Periodicals, Inc.
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