In vivo imaging with a water immersion objective affects brain temperature, blood flow and oxygenation.
mouse
neuroscience
temperature
two-photon microscopy
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
09 08 2019
09 08 2019
Historique:
received:
02
04
2019
accepted:
08
08
2019
pubmed:
10
8
2019
medline:
14
2
2020
entrez:
10
8
2019
Statut:
epublish
Résumé
Previously, we reported the first oxygen partial pressure (Po2) measurements in the brain of awake mice, by performing two-photon phosphorescence lifetime microscopy at micrometer resolution (Lyons et al., 2016). However, this study disregarded that imaging through a cranial window lowers brain temperature, an effect capable of affecting cerebral blood flow, the properties of the oxygen sensors and thus Po2 measurements. Here, we show that in awake mice chronically implanted with a glass window over a craniotomy or a thinned-skull surface, the postsurgical decrease of brain temperature recovers within a few days. However, upon imaging with a water immersion objective at room temperature, brain temperature decreases by ~2-3°C, causing drops in resting capillary blood flow, capillary Po2, hemoglobin saturation, and tissue Po2. These adverse effects are corrected by heating the immersion objective or avoided by imaging through a dry air objective, thereby revealing the physiological values of brain oxygenation.
Identifiants
pubmed: 31397668
doi: 10.7554/eLife.47324
pii: 47324
pmc: PMC6707784
doi:
pii:
Substances chimiques
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : Imaging-in-the-Magnet
Pays : International
Organisme : Swiss National Science Foundation
ID : 310030_182703
Pays : Switzerland
Organisme : Leducq Foundation
ID : Understanding the role of the perivascular space in cerebral small vessel disease
Pays : International
Organisme : ERC
ID : Imaging-in-the-Magnet
Pays : International
Informations de copyright
© 2019, Roche et al.
Déclaration de conflit d'intérêts
MR, EC, RR, DB, BW, SC No competing interests declared
Références
Chemphyschem. 2008 Aug 25;9(12):1673-9
pubmed: 18663708
Elife. 2016 Feb 02;5:
pubmed: 26836304
Cell Metab. 2019 Mar 5;29(3):736-744.e7
pubmed: 30686745
J Neurophysiol. 2015 Jul;114(1):309-22
pubmed: 25972579
Neurophotonics. 2016 Oct;3(4):045007
pubmed: 27981063
J Neurosci. 2011 Sep 21;31(38):13676-81
pubmed: 21940458
Nat Med. 2013 Feb;19(2):241-6
pubmed: 23314058
J Cereb Blood Flow Metab. 2012 Mar;32(3):437-42
pubmed: 22167237
Nat Med. 2011 Jun 05;17(7):893-8
pubmed: 21642977
Cell Rep. 2015 Jul 21;12(3):525-34
pubmed: 26166563
J Cereb Blood Flow Metab. 2011 Apr;31(4):1051-63
pubmed: 21179069
Nat Methods. 2010 Dec;7(12):981-4
pubmed: 20966916
J Neurophysiol. 2012 Dec;108(11):3138-46
pubmed: 22972953
J Comp Physiol B. 2009 Aug;179(6):701-10
pubmed: 19277681
Adv Exp Med Biol. 2011;701:53-9
pubmed: 21445769
Neurophotonics. 2016 Oct;3(4):045005
pubmed: 27774493
Ann Biomed Eng. 2010 Apr;38(4):1683-701
pubmed: 20162361
J Neurophysiol. 2016 Sep 1;116(3):1012-23
pubmed: 27281749
Biom J. 2008 Jun;50(3):346-63
pubmed: 18481363
Nat Commun. 2014 Dec 08;5:5734
pubmed: 25483924
Nat Methods. 2010 Sep;7(9):755-9
pubmed: 20693997
Brain Res Brain Res Protoc. 1998 Sep;3(1):14-21
pubmed: 9767083
J Appl Physiol (1985). 2017 May 1;122(5):1313-1320
pubmed: 28235861
Biomed Opt Express. 2016 Oct 06;7(11):4450-4471
pubmed: 27895987
Nat Protoc. 2018 Jun;13(6):1377-1402
pubmed: 29844521
J Phys Chem A. 2007 Aug 2;111(30):6977-90
pubmed: 17608457
Cell Rep. 2018 Jul 31;24(5):1243-1253.e5
pubmed: 30067979
Opt Express. 2009 Aug 3;17(16):13904-17
pubmed: 19654798
J Biomed Opt. 2018 Nov;24(3):1-11
pubmed: 30468045
Sci Rep. 2018 May 29;8(1):8219
pubmed: 29844478