Simultaneous multisite detection of quantal release from PC12 cells using micro graphitic-diamond multi electrode arrays.
Diamond MEA
Dopamine
L-Dopa
PC12 cells
Secretion
Journal
Biophysical chemistry
ISSN: 1873-4200
Titre abrégé: Biophys Chem
Pays: Netherlands
ID NLM: 0403171
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
29
06
2019
revised:
29
07
2019
accepted:
29
07
2019
pubmed:
10
8
2019
medline:
19
11
2019
entrez:
10
8
2019
Statut:
ppublish
Résumé
Micro graphitic - diamond - multi electrode arrays (μG-D-MEAs) are suitable for measuring multisite quantal dopamine (DA) release from PC12 cells. Following cell stimulation with high extracellular KCl and electrode polarization at +650 mV, amperometric spikes are detected with a mean frequency of 0.60 ± 0.16 Hz. In each recording, simultaneous detection of secretory events is occurred in approximately 50% of the electrodes. Kinetic spike parameters and background noise are preserved among the different electrodes. Comparing the amperometric spikes recorder under control conditions with those recorders from PC12 cells previously incubated for 30 min with the dopamine precursor Levodopa (L-DOPA, 20 μM) it appears that the quantal size of amperometric spikes is increased by 250% and the half-time width (t
Identifiants
pubmed: 31398633
pii: S0301-4622(19)30282-0
doi: 10.1016/j.bpc.2019.106241
pii:
doi:
Substances chimiques
Diamond
7782-40-3
Graphite
7782-42-5
Dopamine
VTD58H1Z2X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
106241Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.