Semi-preparative separation of dihydromyricetin enantiomers by supercritical fluid chromatography and determination of anti-inflammatory activities.
Anti-inflammatory activity
Chromatographic conditions
Dihydromyricetin
Semi-preparative separation
Supercritical fluid chromatography
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
22 Nov 2019
22 Nov 2019
Historique:
received:
12
12
2018
revised:
15
07
2019
accepted:
17
07
2019
pubmed:
12
8
2019
medline:
14
1
2020
entrez:
12
8
2019
Statut:
ppublish
Résumé
Dihydromyricetin, extracted from Ampelopsis grossedentata, has been widely used as one of Chinese health products in recent years. However, limited chiral separation method hinders the studies of pharmacological and pharmacokinetic activity differences of (+)-dihydromyricetin, (-)-dihydromyricetin, and (±)-dihydromyricetin. Herein, we developed a supercritical fluid chromatography approach for chiral separation of dihydromyricetin. Firstly, effects of chiral stationary phase, co-solvent, and flow rate of mobile phase have been investigated in detail. The resolution of 5.11 was achieved for dihydromyricetin enantiomers on amylose tris(3, 5-dimethylphenylcarbamate)-coated chiral stationary phase with the CO
Identifiants
pubmed: 31400842
pii: S0021-9673(19)30770-8
doi: 10.1016/j.chroma.2019.460386
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Flavonols
0
Solvents
0
dihydromyricetin
KD8QND6427
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
460386Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.