Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways.
Animals
Antioxidants
/ metabolism
Caenorhabditis elegans
Caenorhabditis elegans Proteins
/ metabolism
DNA-Binding Proteins
/ metabolism
Flavonoids
/ analysis
Forkhead Transcription Factors
/ metabolism
Longevity
/ drug effects
Malpighiales
/ chemistry
Oxidative Stress
/ drug effects
Phenols
/ analysis
Plant Extracts
/ pharmacology
Plant Leaves
/ chemistry
Plants, Medicinal
Reactive Oxygen Species
/ metabolism
Signal Transduction
/ drug effects
Transcription Factors
/ metabolism
Aging
Antioxidants
Caenorhabditis elegans
DAF-16/FoxO
Glochidion zeylanicum
SKN-1/Nrf2
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
ISSN: 1618-095X
Titre abrégé: Phytomedicine
Pays: Germany
ID NLM: 9438794
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
31
05
2019
revised:
23
07
2019
accepted:
29
07
2019
pubmed:
12
8
2019
medline:
3
3
2020
entrez:
12
8
2019
Statut:
ppublish
Résumé
Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported. We aimed to provide the first science-based evidence of the beneficial effects of GZ on anti-aging and oxidative stress resistance in the Caenorhabditis elegans model. The phytochemical composition of the hexane and methanol extracts were analyzed using GLC-MS and LC-MS. Fingerprinting analysis of the extract was performed by RP-HPLC. We determined total phenolics, flavonoids, and antioxidant properties via DPPH and ABTS assays. Oxidative stress resistance, anti-aging and lifespan were studied in C. elegans treated with leaf extracts. GZ leaf extracts protected the worms against oxidative stress and attenuated ROS accumulation. The expression of stress-response genes, such as SOD-3, and GST-4 were up-regulated, whereas HSP-16.2 was down-regulated after GZ treatment. The oxidative stress resistance properties of GZ possibly involved the DAF-16/FoxO and SKN-1/Nrf-2 transcription factors. GZ leaf extracts improved pharyngeal pumping function and autofluorescent pigment attenuation suggesting anti-aging properties. GZ leaf extracts modulated the lifespan extension in C. elegans. This study reports novel anti-aging and antioxidant activities of GZ leaf extracts, suggesting a novel bioactivity for a medicinally important plant and supplementary drug against oxidative stress.
Sections du résumé
BACKGROUND
BACKGROUND
Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported.
PURPOSE
OBJECTIVE
We aimed to provide the first science-based evidence of the beneficial effects of GZ on anti-aging and oxidative stress resistance in the Caenorhabditis elegans model.
METHODS
METHODS
The phytochemical composition of the hexane and methanol extracts were analyzed using GLC-MS and LC-MS. Fingerprinting analysis of the extract was performed by RP-HPLC. We determined total phenolics, flavonoids, and antioxidant properties via DPPH and ABTS assays. Oxidative stress resistance, anti-aging and lifespan were studied in C. elegans treated with leaf extracts.
RESULTS
RESULTS
GZ leaf extracts protected the worms against oxidative stress and attenuated ROS accumulation. The expression of stress-response genes, such as SOD-3, and GST-4 were up-regulated, whereas HSP-16.2 was down-regulated after GZ treatment. The oxidative stress resistance properties of GZ possibly involved the DAF-16/FoxO and SKN-1/Nrf-2 transcription factors. GZ leaf extracts improved pharyngeal pumping function and autofluorescent pigment attenuation suggesting anti-aging properties. GZ leaf extracts modulated the lifespan extension in C. elegans.
CONCLUSION
CONCLUSIONS
This study reports novel anti-aging and antioxidant activities of GZ leaf extracts, suggesting a novel bioactivity for a medicinally important plant and supplementary drug against oxidative stress.
Identifiants
pubmed: 31401497
pii: S0944-7113(19)30227-2
doi: 10.1016/j.phymed.2019.153061
pii:
doi:
Substances chimiques
Antioxidants
0
Caenorhabditis elegans Proteins
0
DNA-Binding Proteins
0
Flavonoids
0
Forkhead Transcription Factors
0
Phenols
0
Plant Extracts
0
Reactive Oxygen Species
0
Transcription Factors
0
daf-16 protein, C elegans
0
skn-1 protein, C elegans
148733-36-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
153061Informations de copyright
Copyright © 2019. Published by Elsevier GmbH.