Network dynamics of the yeast methyltransferome.
COMPASS
SAM methyltransferase
genetic interaction
histone methyltransferase
phospholipid
Journal
Microbial cell (Graz, Austria)
ISSN: 2311-2638
Titre abrégé: Microb Cell
Pays: Austria
ID NLM: 101632887
Informations de publication
Date de publication:
09 Jul 2019
09 Jul 2019
Historique:
entrez:
13
8
2019
pubmed:
14
8
2019
medline:
14
8
2019
Statut:
epublish
Résumé
Sulfur assimilation and the biosynthesis of methionine, cysteine and S-adenosylmethionine (SAM) are critical to life. As a cofactor, SAM is required for the activity of most methyltransferases (MTases) and as such has broad impact on diverse cellular processes. Assigning function to MTases remains a challenge however, as many MTases are partially redundant, they often have multiple cellular roles and these activities can be condition-dependent. To address these challenges, we performed a systematic synthetic genetic analysis of all pairwise MTase double mutations in normal and stress conditions (16°C, 37°C, and LiCl) resulting in an unbiased comprehensive overview of the complexity and plasticity of the methyltransferome. Based on this network, we performed biochemical analysis of members of the histone H3K4 COMPASS complex and the phospholipid methyltransferase OPI3 to reveal a new role for a phospholipid methyltransferase in mediating histone methylation (H3K4) which underscores a potential link between lipid homeostasis and histone methylation. Our findings provide a valuable resource to study methyltransferase function, the dynamics of the methyltransferome, genetic crosstalk between biological processes and the dynamics of the methyltransferome in response to cellular stress.
Identifiants
pubmed: 31403050
doi: 10.15698/mic2019.08.687
pii: MIC0179E126
pmc: PMC6685046
doi:
Types de publication
Journal Article
Langues
eng
Pagination
356-369Déclaration de conflit d'intérêts
Conflict of interest: The authors declare that they have no conflict of interests.
Références
Biochem Biophys Res Commun. 2000 Jul 21;274(1):105-11
pubmed: 10903903
Mol Biol Cell. 2000 Dec;11(12):4241-57
pubmed: 11102521
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):12902-7
pubmed: 11687631
Science. 2001 Dec 14;294(5550):2364-8
pubmed: 11743205
Nature. 2002 Jul 25;418(6896):387-91
pubmed: 12140549
Curr Opin Struct Biol. 2002 Dec;12(6):783-93
pubmed: 12504684
Trends Biochem Sci. 2003 Jun;28(6):329-35
pubmed: 12826405
Science. 2004 Feb 6;303(5659):808-13
pubmed: 14764870
Mol Cell Biol. 2004 Apr;24(7):2932-43
pubmed: 15024081
Nat Rev Genet. 2004 Nov;5(11):826-37
pubmed: 15520792
Cell. 2005 Nov 4;123(3):507-19
pubmed: 16269340
Biochem J. 2006 May 1;395(3):563-70
pubmed: 16426232
Genome Biol. 2006;7(7):R63
pubmed: 16859555
J Biol Chem. 2006 Nov 17;281(46):35404-12
pubmed: 16921172
Genetics. 2006 Nov;174(3):1135-49
pubmed: 16951088
Nat Genet. 2007 Feb;39(2):199-206
pubmed: 17206143
Nature. 2007 Apr 12;446(7137):806-10
pubmed: 17314980
Plant Cell. 2008 Jan;20(1):186-200
pubmed: 18223037
PLoS Comput Biol. 2008 Apr 18;4(4):e1000065
pubmed: 18421374
Nat Chem Biol. 2008 Jun;4(6):344-6
pubmed: 18438403
Science. 2008 Oct 17;322(5900):405-10
pubmed: 18818364
Cell. 2009 Mar 6;136(5):952-63
pubmed: 19269370
Genetics. 2009 Jun;182(2):623-6
pubmed: 19332878
Mol Cell Proteomics. 2009 Jul;8(7):1516-26
pubmed: 19351663
Science. 2010 Jan 22;327(5964):425-31
pubmed: 20093466
J Biol Chem. 2010 Mar 26;285(13):9636-41
pubmed: 20118233
Methods Enzymol. 2010;470:145-79
pubmed: 20946810
Methods Enzymol. 2010;470:205-31
pubmed: 20946812
Nat Methods. 2010 Dec;7(12):1017-24
pubmed: 21076421
Mol Syst Biol. 2010 Nov 30;6:430
pubmed: 21119626
Science. 2010 Dec 3;330(6009):1385-9
pubmed: 21127252
PLoS One. 2011;6(8):e23168
pubmed: 21858014
Methods Mol Biol. 2011;759:239-69
pubmed: 21863492
Mol Biol Cell. 2011 Nov;22(21):4192-204
pubmed: 21900497
PLoS Genet. 2011 Nov;7(11):e1002353
pubmed: 22102822
Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20526-31
pubmed: 22158900
Science. 2013 Jan 11;339(6116):215-8
pubmed: 23160953
Chembiochem. 2012 Dec 21;13(18):2642-55
pubmed: 23180741
Mol Biol Cell. 2014 May;25(10):1653-65
pubmed: 24648496
Science. 2014 Apr 11;344(6180):208-11
pubmed: 24723613
Cell Mol Life Sci. 2015 Jun;72(11):2041-59
pubmed: 25662273
Nat Rev Cancer. 2015 Mar;15(3):152-65
pubmed: 25693834
Science. 2016 Sep 23;353(6306):
pubmed: 27708008
Mol Cell. 2017 Apr 20;66(2):180-193.e8
pubmed: 28366644
Science. 2018 Apr 20;360(6386):null
pubmed: 29674565
Nucleic Acids Res. 2019 Jan 8;47(D1):D529-D541
pubmed: 30476227
Mutat Res. 1974 Sep;24(3):281-92
pubmed: 4606119
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
pubmed: 9843981