Photoirradiation after aminolevulinic acid treatment suppresses cancer cell proliferation through the HO-1/p21 pathway.
Aminolevulinic Acid
/ pharmacology
Cell Line, Tumor
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Cyclin-Dependent Kinase Inhibitor p21
/ metabolism
Gene Expression
HEK293 Cells
/ drug effects
Heme Oxygenase-1
/ metabolism
Humans
PC-3 Cells
/ drug effects
Photochemotherapy
/ methods
Photosensitizing Agents
/ pharmacology
RNA, Messenger
/ metabolism
Reactive Oxygen Species
/ metabolism
Stomach Neoplasms
/ drug therapy
Up-Regulation
/ drug effects
5-aminolevulinic acid
Cancer
Cell cycle
Photodynamic therapy
p21
Journal
Photodiagnosis and photodynamic therapy
ISSN: 1873-1597
Titre abrégé: Photodiagnosis Photodyn Ther
Pays: Netherlands
ID NLM: 101226123
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
05
06
2019
revised:
09
07
2019
accepted:
26
07
2019
pubmed:
14
8
2019
medline:
2
5
2020
entrez:
13
8
2019
Statut:
ppublish
Résumé
Photodynamic therapy (PDT) and diagnosis (PDD) using 5-aminolevulinic acid (ALA) to control the production of an intracellular photosensitizer, protoporphyrin IX (PpIX), are in common clinical use. Although various studies have been published regarding cell death analysis after photoirradiation by ALA-PDT, the changes in gene expressions induced by it are yet unclear. Here, we focused on studying gene expression and cell proliferation changes in cancer cells that survive photoirradiation. HEK293 human embryonic kidney cells, MKN45 human gastric cells, and PC-3 human prostate cancer cells were selected for this research. Cell viability was measured using trypan blue and MTT assays. ALA-PDT experiments were performed using a calibrated LED irradiation module. Furthermore, mRNA and protein gene expression analysis were performed using our previously reported methods. mRNAs of PAI-1, HO-1, and p21 were upregulated after photoirradiation of HEK293, which was suppressed by N-acetyl-L-cysteine, a reactive oxygen species (ROS) scavenger. Primer array results in PC-3 cells and p21 and Ki-67 expression results in both PC-3 and MKN45 cells suggested that photoirradiation suppressed cell proliferation. Cell numbers post-photoirradiation revealed that the proliferation of surviving cells was suppressed in PC-3 and MKN45 cells. ALA-PDD or ALA-PDT can result in rapid ROS-induced cell death and may decrease long-term recurrence rates through several pathways including the HO-1/p21 pathway.
Sections du résumé
BACKGROUND
BACKGROUND
Photodynamic therapy (PDT) and diagnosis (PDD) using 5-aminolevulinic acid (ALA) to control the production of an intracellular photosensitizer, protoporphyrin IX (PpIX), are in common clinical use. Although various studies have been published regarding cell death analysis after photoirradiation by ALA-PDT, the changes in gene expressions induced by it are yet unclear. Here, we focused on studying gene expression and cell proliferation changes in cancer cells that survive photoirradiation.
METHODS
METHODS
HEK293 human embryonic kidney cells, MKN45 human gastric cells, and PC-3 human prostate cancer cells were selected for this research. Cell viability was measured using trypan blue and MTT assays. ALA-PDT experiments were performed using a calibrated LED irradiation module. Furthermore, mRNA and protein gene expression analysis were performed using our previously reported methods.
RESULTS
RESULTS
mRNAs of PAI-1, HO-1, and p21 were upregulated after photoirradiation of HEK293, which was suppressed by N-acetyl-L-cysteine, a reactive oxygen species (ROS) scavenger. Primer array results in PC-3 cells and p21 and Ki-67 expression results in both PC-3 and MKN45 cells suggested that photoirradiation suppressed cell proliferation. Cell numbers post-photoirradiation revealed that the proliferation of surviving cells was suppressed in PC-3 and MKN45 cells.
CONCLUSION
CONCLUSIONS
ALA-PDD or ALA-PDT can result in rapid ROS-induced cell death and may decrease long-term recurrence rates through several pathways including the HO-1/p21 pathway.
Identifiants
pubmed: 31404677
pii: S1572-1000(19)30284-4
doi: 10.1016/j.pdpdt.2019.07.021
pii:
doi:
Substances chimiques
Cyclin-Dependent Kinase Inhibitor p21
0
Photosensitizing Agents
0
RNA, Messenger
0
Reactive Oxygen Species
0
Aminolevulinic Acid
88755TAZ87
Heme Oxygenase-1
EC 1.14.14.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10-17Informations de copyright
Copyright © 2019. Published by Elsevier B.V.