Gene Therapy for Parkinson's Disease: Preclinical Evaluation of Optimally Configured TH:CH1 Fusion for Maximal Dopamine Synthesis.

MPTP primate model Parkinson’s disease dopamine replacement gene therapy lentiviral vector

Journal

Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857

Informations de publication

Date de publication:
13 Sep 2019
Historique:
received: 19 11 2018
accepted: 08 07 2019
entrez: 14 8 2019
pubmed: 14 8 2019
medline: 14 8 2019
Statut: epublish

Résumé

A recent phase I-II, open-label trial of ProSavin, a lentiviral vector delivering the key enzymes in the dopamine biosynthetic pathway to non-dopaminergic striatal neurons, demonstrated safety and improved motor function in parkinsonian patients. However, the magnitude of the effect suggested that optimal levels of dopamine replacement may not have been achieved. OXB-102, a lentiviral vector with an optimized expression cassette for dopamine biosynthesis, has been shown to achieve a significantly higher dopamine yield than ProSavin. We assessed the efficacy of OXB-102 in the MPTP macaque model of Parkinson's disease (PD). At 6 months post-vector administration, all treated animals showed significant improvements in clinical scores and spontaneous locomotor activity compared to controls, with the highest recovery observed in the OXB-102 high-dose (HD) group. Positron emission tomography quantification of 6-[

Identifiants

pubmed: 31406701
doi: 10.1016/j.omtm.2019.07.002
pii: S2329-0501(19)30073-7
pmc: PMC6685641
doi:

Types de publication

Journal Article

Langues

eng

Pagination

206-216

Références

Synapse. 1999 Nov;34(2):111-23
pubmed: 10502310
Curr Pharm Des. 2000 Aug;6(12):1211-48
pubmed: 10903391
Gene Ther. 2002 Mar;9(6):381-9
pubmed: 11960314
J Neurosci. 2002 Dec 1;22(23):10302-12
pubmed: 12451130
CMAJ. 2003 Feb 4;168(3):293-301
pubmed: 12566335
Neuron. 2003 Sep 11;39(6):889-909
pubmed: 12971891
Mol Ther. 2007 Jan;15(1):62-8
pubmed: 17164776
Am Fam Physician. 2006 Dec 15;74(12):2046-54
pubmed: 17186710
Lancet. 2007 Jun 23;369(9579):2097-105
pubmed: 17586305
Mov Disord. 2008 Apr 15;23(5):690-9
pubmed: 18228568
Lancet Neurol. 2008 May;7(5):400-8
pubmed: 18387850
Front Hum Neurosci. 2008 Mar 28;1:9
pubmed: 18958223
Sci Transl Med. 2009 Oct 14;1(2):2ra4
pubmed: 20368163
Lancet Neurol. 2010 Dec;9(12):1164-1172
pubmed: 20970382
Lancet Neurol. 2011 Apr;10(4):309-19
pubmed: 21419704
Annu Rev Neurosci. 2011;34:441-66
pubmed: 21469956
Mol Ther. 2012 Jul;20(7):1315-26
pubmed: 22294150
Hum Gene Ther. 2012 Apr;23(4):377-81
pubmed: 22424171
Mov Disord. 2012 Sep 15;27(11):1373-8
pubmed: 22976821
Invest Ophthalmol Vis Sci. 2013 Jun 12;54(6):4061-71
pubmed: 23620430
Behav Brain Res. 2013 Aug 1;250:199-205
pubmed: 23651878
Lancet. 2014 Mar 29;383(9923):1138-46
pubmed: 24412048
Front Neurol. 2014 Dec 15;5:242
pubmed: 25566170
J Labelled Comp Radiopharm. 2015 Jun 15;58(7):281-90
pubmed: 26011311
Ann Neurol. 2015 Aug;78(2):248-57
pubmed: 26061140
J Mov Disord. 2015 May;8(2):92-7
pubmed: 26090081
Hum Gene Ther. 2016 Jul;27(7):522-7
pubmed: 26711317
Hum Gene Ther Clin Dev. 2016 Sep;27(3):100-10
pubmed: 27470285
Front Aging Neurosci. 2017 May 03;9:126
pubmed: 28515689
Curr Pharmacol Rep. 2016 Dec;2(6):253-261
pubmed: 28534003
Hum Gene Ther. 2017 Nov;28(11):988-1003
pubmed: 29035118
Mov Disord. 2018 Jul;33(6):859-866
pubmed: 29178365
Hum Gene Ther Clin Dev. 2018 Sep;29(3):148-155
pubmed: 30156440
Ann Neurol. 2019 May;85(5):704-714
pubmed: 30802998
Adv Neurol. 1993;60:626-35
pubmed: 8093582
Clin Neuropharmacol. 1994;17 Suppl 2:S32-44
pubmed: 9358193

Auteurs

Romina Aron Badin (RA)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Katie Binley (K)

Oxford Biomedica, Windrush Court, Transport Way, Oxford OX4 6LT, UK.

Nadja Van Camp (N)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Caroline Jan (C)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Jeanne Gourlay (J)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Camille Robert (C)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Pauline Gipchtein (P)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Audrey Fayard (A)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Hannah Stewart (H)

Oxford Biomedica, Windrush Court, Transport Way, Oxford OX4 6LT, UK.

G Scott Ralph (GS)

Oxford Biomedica, Windrush Court, Transport Way, Oxford OX4 6LT, UK.

Yatish Lad (Y)

Oxford Biomedica, Windrush Court, Transport Way, Oxford OX4 6LT, UK.

Michelle Kelleher (M)

Oxford Biomedica, Windrush Court, Transport Way, Oxford OX4 6LT, UK.

Julie Loader (J)

Oxford Biomedica, Windrush Court, Transport Way, Oxford OX4 6LT, UK.

Koichi Hosomi (K)

AP-HP, Groupe Hospitalier Henri-Mondor, DHU PePsy, Neurochirurgie, Créteil, 94010, France.
Université Paris 12, Faculté de Médecine, IMRB, INSERM U955, Team 14, Créteil 94010, France.

Stéphane Palfi (S)

AP-HP, Groupe Hospitalier Henri-Mondor, DHU PePsy, Neurochirurgie, Créteil, 94010, France.
Université Paris 12, Faculté de Médecine, IMRB, INSERM U955, Team 14, Créteil 94010, France.

Kyriacos A Mitrophanous (KA)

Oxford Biomedica, Windrush Court, Transport Way, Oxford OX4 6LT, UK.

Philippe Hantraye (P)

CEA, DRF, Institute of Biology François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France.
CNRS, CEA, Paris-Sud University, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Classifications MeSH