Identification of a novel protease from the thermophilic Anoxybacillus kamchatkensis M1V and its application as laundry detergent additive.

Anoxybacillus / enzymology Bacterial Proteins / chemistry Detergents / chemistry Enzyme Stability Hot Temperature Peptide Hydrolases / chemistry Recombinant Proteins / chemistry
Anoxybacillus kamchatkensis Detergent Expression Hot spring Protease

Journal

Extremophiles : life under extreme conditions
ISSN: 1433-4909
Titre abrégé: Extremophiles
Pays: Germany
ID NLM: 9706854

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 04 04 2019
accepted: 26 07 2019
pubmed: 14 8 2019
medline: 28 2 2020
entrez: 14 8 2019
Statut: ppublish

Résumé

A thermostable extracellular alkaline protease (called SAPA) was produced (4600 U/mL) by Anoxybacillus kamchatkensis M1V, purified to homogeneity, and biochemically characterized. SAPA is a monomer with a molecular mass of 28 kDa estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Native-PAGE, casein-zymography, and size exclusion using high performance liquid chromatography (HPLC). The sequence of its NH

Identifiants

DOI: 10.1007/s00792-019-01123-6 PMID: 31407121
pubmed: 31407121
doi: 10.1007/s00792-019-01123-6
pii: 10.1007/s00792-019-01123-6
doi:

Substances chimiques

Bacterial Proteins 0
Detergents 0
Recombinant Proteins 0
Peptide Hydrolases EC 3.4.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

687-706

Subventions

Organisme : Direction Générale de la Recherche Scientifique et du Développement Technologique
ID : Contract program grant n°.: LMBEE-CBS/code: LR15C

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Auteurs

Sondes Mechri (S)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Khelifa Bouacem (K)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), El Alia, P.O. Box 32, 16111, Bab Ezzouar, Algiers, Algeria.

Nadia Zaraî Jaouadi (N)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
Biotech ECOZYM Start-Up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Hatem Rekik (H)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
Biotech ECOZYM Start-Up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Mouna Ben Elhoul (M)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
Biotech ECOZYM Start-Up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Maroua Omrane Benmrad (M)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Hocine Hacene (H)

Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), El Alia, P.O. Box 32, 16111, Bab Ezzouar, Algiers, Algeria.

Samir Bejar (S)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
Biotech ECOZYM Start-Up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Amel Bouanane-Darenfed (A)

Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), El Alia, P.O. Box 32, 16111, Bab Ezzouar, Algiers, Algeria.

Bassem Jaouadi (B)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia. bassem.jaouadi@cbs.rnrt.tn.
Biotech ECOZYM Start-Up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia. bassem.jaouadi@cbs.rnrt.tn.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Anoxybacillus › Identification of a novel protease from the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Identification of a novel protease from the...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits domestiques › Détergents › Identification of a novel protease from the...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Identification of a novel protease from the...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Température élevée › Identification of a novel protease from the...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Identification of a novel protease from the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Identification of a novel protease from the...