Quantitative CE analysis of punicalagin in Combretum aculeatum extracts traditionally used in Senegal for the treatment of tuberculosis.


Journal

Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476

Informations de publication

Date de publication:
11 2019
Historique:
received: 15 01 2019
revised: 26 07 2019
accepted: 10 08 2019
pubmed: 14 8 2019
medline: 31 3 2020
entrez: 14 8 2019
Statut: ppublish

Résumé

Mycobacterium tuberculosis is the causative agent of tuberculosis, an infectious bacterial disease, which most commonly affects the lungs. In the search for novel active compounds or medicines against tuberculosis, an ethnopharmacological survey combined with a host-pathogen assay has recently highlighted the potency of an aqueous extract of Combretum aculeatum. C. aculeatum is used in traditional medicine and has demonstrated a significant in vitro antimycobacterial activity. Punicalagin, an ellagitannin, was isolated and found to be related to the biological activity of the extract. An analytical method for the evaluation of punicalagin in C. aculeatum was developed by capillary electrophoresis. After method optimization, the quantification of punicalagin was achieved for the evaluation of various plant extracts to determine the content of punicalagin related to the extraction modes and conditions, origin of the plant material, and harvesting period. The developed method demonstrated that the leaves presented the highest punicalagin content compared to the seeds and stems. A decoction of 30 min in boiling water was found to be the best extraction mode of C. aculeatum.

Identifiants

pubmed: 31407800
doi: 10.1002/elps.201900240
doi:

Substances chimiques

Antitubercular Agents 0
Hydrolyzable Tannins 0
Plant Extracts 0
punicalagin 65995-63-3

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2820-2827

Informations de copyright

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Auteurs

ElHadji Assane Diop (EA)

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.
Biology Department, Université Cheikh Anta Diop, Dakar, Senegal.

Jenna Jacquat (J)

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.

Nicolas Drouin (N)

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.

Emerson Ferreira Queiroz (EF)

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.

Jean-Luc Wolfender (JL)

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.

Tahir Diop (T)

Biology Department, Université Cheikh Anta Diop, Dakar, Senegal.

Julie Schappler (J)

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.

Serge Rudaz (S)

School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.

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