Monosynaptic tracing: a step-by-step protocol.

Brain / metabolism Neuroanatomical Tract-Tracing Techniques / methods Neuronal Tract-Tracers Neurons / metabolism Rabies virus

Journal

Journal of chemical neuroanatomy
ISSN: 1873-6300
Titre abrégé: J Chem Neuroanat
Pays: Netherlands
ID NLM: 8902615

Informations de publication

Date de publication:
12 2019
Historique:
received: 17 03 2019
revised: 01 07 2019
accepted: 14 07 2019
pubmed: 14 8 2019
medline: 15 9 2020
entrez: 14 8 2019
Statut: ppublish

Résumé

Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.

Identifiants

DOI: 10.1016/j.jchemneu.2019.101661 PMID: 31408693
pubmed: 31408693
pii: S0891-0618(19)30056-0
doi: 10.1016/j.jchemneu.2019.101661
pii:
doi:

Substances chimiques

Neuronal Tract-Tracers 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

101661

Subventions

Organisme : NIMH NIH HHS
ID : U19 MH114830
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH114829
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH106018
Pays : United States

Informations de copyright

Copyright © 2019. Published by Elsevier B.V.

Auteurs

Thomas K Lavin (TK)

McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States.

Lei Jin (L)

McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States.

Ian R Wickersham (IR)

McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States. Electronic address: wickersham@mit.edu.

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Classifications MeSH

questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Monosynaptic tracing: a step-by-step protocol.
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Techniques de traçage neuroanatomique › Monosynaptic tracing: a step-by-step protocol.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Traceurs neuronaux › Monosynaptic tracing: a step-by-step protocol.
questionsmedicales.fr › Cellules › Neurones › Monosynaptic tracing: a step-by-step protocol.
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Mononegavirales › Rhabdoviridae › Lyssavirus › Virus de la rage › Monosynaptic tracing: a step-by-step protocol.