Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 08 2019
13 08 2019
Historique:
received:
10
01
2019
accepted:
26
07
2019
entrez:
15
8
2019
pubmed:
15
8
2019
medline:
20
11
2020
Statut:
epublish
Résumé
In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the "heavy duty" carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
Identifiants
pubmed: 31409839
doi: 10.1038/s41598-019-48037-z
pii: 10.1038/s41598-019-48037-z
pmc: PMC6692331
doi:
Substances chimiques
Tretinoin
5688UTC01R
Cytochromes c
9007-43-6
Ferritins
9007-73-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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