An alternative framework for fluorescence correlation spectroscopy.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 08 2019
14 08 2019
Historique:
received:
30
03
2018
accepted:
11
07
2019
entrez:
16
8
2019
pubmed:
16
8
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Fluorescence correlation spectroscopy (FCS), is a widely used tool routinely exploited for in vivo and in vitro applications. While FCS provides estimates of dynamical quantities, such as diffusion coefficients, it demands high signal to noise ratios and long time traces, typically in the minute range. In principle, the same information can be extracted from microseconds to seconds long time traces; however, an appropriate analysis method is missing. To overcome these limitations, we adapt novel tools inspired by Bayesian non-parametrics, which starts from the direct analysis of the observed photon counts. With this approach, we are able to analyze time traces, which are too short to be analyzed by existing methods, including FCS. Our new analysis extends the capability of single molecule fluorescence confocal microscopy approaches to probe processes several orders of magnitude faster and permits a reduction of photo-toxic effects on living samples induced by long periods of light exposure.
Identifiants
pubmed: 31413259
doi: 10.1038/s41467-019-11574-2
pii: 10.1038/s41467-019-11574-2
pmc: PMC6694112
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3662Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM121885
Pays : United States
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