Long-term observation of Magnetospirillum gryphiswaldense in a microfluidic channel.
Bacteria
Magneto-tactic
Microfluidic
Velocity
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
21
02
2019
accepted:
02
08
2019
revised:
22
07
2019
pubmed:
16
8
2019
medline:
16
11
2019
entrez:
16
8
2019
Statut:
ppublish
Résumé
We controlled and observed individual magneto-tactic bacteria (Magnetospirillum gryphiswaldense) inside a [Formula: see text]-high microfluidic channel for over 4 h. After a period of constant velocity, the duration of which varied between bacteria, all observed bacteria showed a gradual decrease in their velocity of about [Formula: see text]. After coming to a full stop, different behaviour was observed, ranging from rotation around the centre of mass synchronous with the direction of the external magnetic field, to being completely immobile. Our results suggest that the influence of the high-intensity illumination and the presence of the channel walls are important parameters to consider when performing observations of such long duration.
Identifiants
pubmed: 31414157
doi: 10.1007/s00203-019-01713-0
pii: 10.1007/s00203-019-01713-0
pmc: PMC6817740
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1427-1433Références
FEMS Immunol Med Microbiol. 2003 Jan 21;35(1):17-24
pubmed: 12589953
Biophys J. 2007 Aug 15;93(4):1402-12
pubmed: 17526564
Biophys J. 2008 Nov 1;95(9):4481-93
pubmed: 18658218
Arch Microbiol. 2013 Jan;195(1):63-74
pubmed: 23090570
Genome Announc. 2018 Apr 26;6(17):null
pubmed: 29700148
Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14861-6
pubmed: 19706420
Biophys J. 2006 Jan 15;90(2):400-12
pubmed: 16239332
Appl Microbiol Biotechnol. 2008 Jun;79(3):389-97
pubmed: 18425510
Biomed Microdevices. 2014 Oct;16(5):761-70
pubmed: 24951158
Environ Microbiol. 2017 Sep;19(9):3638-3648
pubmed: 28752909
Lab Chip. 2001 Sep;1(1):50-5
pubmed: 15100889
Nat Nanotechnol. 2016 Nov;11(11):941-947
pubmed: 27525475
Nat Nanotechnol. 2014 Mar;9(3):193-7
pubmed: 24561353
Phys Rev Lett. 2008 Jul 18;101(3):038102
pubmed: 18764299
Philos Trans R Soc Lond B Biol Sci. 2000 Apr 29;355(1396):473-89
pubmed: 10836501
Biophys J. 1997 Aug;73(2):994-1000
pubmed: 9251816
Biophys J. 2014 Jan 7;106(1):37-46
pubmed: 24411235
Nat Commun. 2015 Nov 02;6:8776
pubmed: 26522289
Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):556-61
pubmed: 24344288
Biophys J. 2014 Jul 15;107(2):527-538
pubmed: 25028894