Proximity Induced Splicing Utilizing Caged Split Inteins.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
04 09 2019
04 09 2019
Historique:
pubmed:
17
8
2019
medline:
22
10
2020
entrez:
17
8
2019
Statut:
ppublish
Résumé
Naturally split inteins drive the ligation of separately expressed polypeptides through a process called protein trans splicing (PTS). The ability to control PTS, so-called conditional protein splicing (CPS), has led to the development of tools to modulate protein structure and function at the post-translational level. CPS applications that utilize proximity as a trigger are especially intriguing as they afford the possibility to activate proteins in both a temporal and spatially targeted manner. In this study, we present the first proximity triggered CPS method that utilizes a naturally split fast splicing intein, Npu. We show that this method is amenable to diverse proximity triggers and capable of reconstituting and locally activating the acetyltransferase p300 in mammalian cells. This technology opens up a range of possibilities for the use of proximity triggered CPS.
Identifiants
pubmed: 31418547
doi: 10.1021/jacs.9b05721
pmc: PMC6903685
mid: NIHMS1054419
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13708-13712Subventions
Organisme : NIGMS NIH HHS
ID : R37 GM086868
Pays : United States
Références
J Am Chem Soc. 2015 Feb 18;137(6):2155-8
pubmed: 25647354
J Biol Chem. 2014 May 23;289(21):14490-7
pubmed: 24695741
J Biol Chem. 2012 Aug 17;287(34):28686-96
pubmed: 22753413
Nat Chem Biol. 2007 Jan;3(1):50-4
pubmed: 17128262
Mol Biosyst. 2014 Apr;10(4):831-7
pubmed: 24481070
Angew Chem Int Ed Engl. 2011 Mar 28;50(14):3249-52
pubmed: 21384476
Anal Chem. 2018 Aug 21;90(16):9779-9786
pubmed: 30028129
Nat Biotechnol. 2015 May;33(5):510-7
pubmed: 25849900
J Biol Chem. 1998 Apr 24;273(17):10567-77
pubmed: 9553117
Angew Chem Int Ed Engl. 2004 Oct 4;43(39):5189-92
pubmed: 15455415
Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8538-8543
pubmed: 28739907
J Am Chem Soc. 2006 Jul 12;128(27):8939-46
pubmed: 16819890
Chem Commun (Camb). 2015 Jun 14;51(47):9670-3
pubmed: 25977944
Chembiochem. 2010 Jul 5;11(10):1368-72
pubmed: 20512791
Nucleic Acids Res. 1998 Nov 15;26(22):5109-15
pubmed: 9801307
Mol Biosyst. 2011 Jun;7(6):2031-9
pubmed: 21487580
Angew Chem Int Ed Engl. 2008;47(40):7764-7
pubmed: 18767096
J Am Chem Soc. 2017 Jun 21;139(24):8074-8077
pubmed: 28562027
J Am Chem Soc. 2013 Dec 11;135(49):18673-81
pubmed: 24236406
Angew Chem Int Ed Engl. 2015 Feb 9;54(7):2116-20
pubmed: 25557882
Gene. 1997 Jun 19;192(2):271-81
pubmed: 9224900
J Am Chem Soc. 2002 Aug 7;124(31):9044-5
pubmed: 12148996
Nat Chem Biol. 2015 May;11(5):316-8
pubmed: 25848930
Cell. 2013 Feb 28;152(5):1173-83
pubmed: 23452860
Biochemistry. 2006 Feb 14;45(6):1571-8
pubmed: 16460004
Nat Methods. 2015 Nov;12(11):1085-90
pubmed: 26389572
PLoS One. 2015 Aug 28;10(8):e0135965
pubmed: 26317656
J Am Chem Soc. 2018 Sep 19;140(37):11791-11799
pubmed: 30156841
J Am Chem Soc. 2016 Feb 24;138(7):2162-5
pubmed: 26854538
Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10505-10
pubmed: 15247421
Nat Methods. 2008 Apr;5(4):303-5
pubmed: 18272963
Chem Sci. 2014;5(1):446-461
pubmed: 24634716
Protein Sci. 2005 Feb;14(2):523-32
pubmed: 15632292
Nat Struct Mol Biol. 2004 Apr;11(4):308-15
pubmed: 15004546
Chem Biol. 2011 May 27;18(5):619-30
pubmed: 21609843