Model of persistent foot-and-mouth disease virus infection in multilayered cells derived from bovine dorsal soft palate.
Animals
Antigens, Viral
/ immunology
Cattle
Cattle Diseases
/ virology
Cell Line
Cells, Cultured
Epithelial Cells
/ virology
Female
Foot-and-Mouth Disease
/ virology
Foot-and-Mouth Disease Virus
/ immunology
Genome, Viral
/ genetics
Immunohistochemistry
/ veterinary
Male
Palate, Soft
/ virology
RNA, Viral
/ analysis
Swine
air-liquid interface models
bovine dorsal soft palate
cattle
epithelial cells
foot-and-mouth disease virus
persistence
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
02
09
2018
revised:
02
08
2019
accepted:
09
08
2019
pubmed:
17
8
2019
medline:
2
5
2020
entrez:
17
8
2019
Statut:
ppublish
Résumé
Foot-and-mouth disease virus (FMDV) causes a highly contagious vesicular disease in livestock, with serious consequences for international trade. The virus persists in the nasopharynx of cattle and this slows down the process to obtain an FMDV-free status after an outbreak. To study biological mechanisms, or to identify molecules that can be targeted to diagnose or interfere with persistence, we developed a model of persistent FMDV infection in bovine dorsal soft palate (DSP). Primary DSP cells were isolated after commercial slaughter and were cultured in multilayers at the air-liquid interface. After 5 weeks of culture without further passage, the cells were infected with FMDV strain O/FRA/1/2001. Approximately, 20% of cells still had a polygonal morphology and displayed tight junctions as in stratified squamous epithelia. Subsets of cells expressed cytokeratin and most or all cells expressed vimentin. In contrast to monolayers in medium, multilayers in air demonstrated only a limited cytopathic effect. Integrin α
Identifiants
pubmed: 31419374
doi: 10.1111/tbed.13332
pmc: PMC7003861
doi:
Substances chimiques
Antigens, Viral
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
133-148Subventions
Organisme : Svenska Forskningsrådet Formas
Organisme : Federal Office for Agriculture and Food (BLE, Germany)
Organisme : Agence Nationale de la Recherche
ID : ANIHWA ERA NET Transcriptovac FP#26
Informations de copyright
© 2019 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.
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