Insights into Pasteurellaceae carriage dynamics in the nasal passages of healthy beef calves.
Animals
Bacterial Load
Carrier State
Cattle
Cattle Diseases
/ diagnosis
DNA, Bacterial
/ genetics
Longitudinal Studies
Male
Mannheimia haemolytica
/ classification
Nasal Cavity
/ microbiology
Pasteurella multocida
/ classification
Pasteurellaceae
/ classification
Pasteurellaceae Infections
/ diagnosis
Polymerase Chain Reaction
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 08 2019
16 08 2019
Historique:
received:
07
02
2019
accepted:
16
07
2019
entrez:
18
8
2019
pubmed:
20
8
2019
medline:
23
10
2020
Statut:
epublish
Résumé
We investigated three bovine respiratory pathobionts in healthy cattle using qPCR optimised and validated to quantify Histophilus somni, Mannheimia haemolytica and Pasteurella multocida over a wide dynamic range. A longitudinal study was conducted to investigate the carriage and density of these bacteria in the nasal passages of healthy beef calves (N = 60) housed over winter in an experimental farm setting. The three pathobiont species exhibited remarkably different carriage rates and density profiles. At housing, high carriage rates were observed for P. multocida (95%), and H. somni (75%), while fewer calves were positive for M. haemolytica (13%). Carriage rates for all three bacterial species declined over the 75-day study, but not all individuals became colonised despite sharing of environment and airspace. Colonisation patterns ranged from continuous to intermittent and were different among pathobiont species. Interval-censored exponential survival models estimated the median duration of H. somni and P. multocida carriage at 14.8 (CI
Identifiants
pubmed: 31420565
doi: 10.1038/s41598-019-48007-5
pii: 10.1038/s41598-019-48007-5
pmc: PMC6697682
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
11943Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/C/000I0320
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/C/000J0100
Pays : United Kingdom
Commentaires et corrections
Type : ErratumIn
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