VEGF and FGF signaling during head regeneration in hydra.
Animals
Computer Simulation
Fibroblast Growth Factor 1
/ chemistry
Fibroblast Growth Factors
/ metabolism
Gene Expression Regulation
Head
/ physiology
Humans
Hydra
/ drug effects
Indoles
/ pharmacology
Protein Domains
Pyrroles
/ pharmacology
Receptor, Fibroblast Growth Factor, Type 1
/ chemistry
Regeneration
/ drug effects
Signal Transduction
Structural Homology, Protein
Vascular Endothelial Growth Factor A
/ metabolism
Vascular Endothelial Growth Factor Receptor-2
/ chemistry
Decapitation
FGF-1
FGFR-1
Head regeneration
Hydra
VEGFR-2
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
30 Oct 2019
30 Oct 2019
Historique:
received:
09
04
2019
revised:
10
08
2019
accepted:
12
08
2019
pubmed:
20
8
2019
medline:
10
9
2019
entrez:
18
8
2019
Statut:
ppublish
Résumé
Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) signaling pathways play important roles in the formation of the blood vascular system and nervous system across animal phyla. We have earlier reported VEGF and FGF from Hydra vulgaris Ind-Pune, a cnidarian with a defined body axis, an organized nervous system and a remarkable ability of regeneration. We have now identified three more components of VEGF and FGF signaling pathways from hydra. These include FGF-1, FGF receptor 1 (FGFR-1) and VEGF receptor 2 (VEGFR-2) with a view to deciphering their possible roles in regeneration. In silico analysis of proteins was performed using Clustal omega, Swiss model, MEGA 7.0, etc. Gene expression was studied by whole mount in situ hybridization. VEGF and FGF signaling was inhibited using specific pharmacological inhibitors and their effects on head regeneration were studied. Expression patterns of the genes indicate a possible interaction between FGF-1 and FGFR-1 and also VEGF and VEGFR-2. Upon treatment of decapitated hydra with pharmacological inhibitor of FGFR-1 or VEGFR-2 for 48 h, head regeneration was delayed in treated as compared to untreated, control regenerates. When we studied the expression of head specific genes HyBra1 and HyKs1 and tentacle specific gene HyAlx in control and treated regenerates using whole mount in situ hybridization, expression of all the three genes was found to be adversely affected in treated regenerates. The results suggest that VEGF and FGF signaling play important roles in regeneration of hypostome and tentacles in hydra.
Sections du résumé
BACKGROUND
BACKGROUND
Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) signaling pathways play important roles in the formation of the blood vascular system and nervous system across animal phyla. We have earlier reported VEGF and FGF from Hydra vulgaris Ind-Pune, a cnidarian with a defined body axis, an organized nervous system and a remarkable ability of regeneration. We have now identified three more components of VEGF and FGF signaling pathways from hydra. These include FGF-1, FGF receptor 1 (FGFR-1) and VEGF receptor 2 (VEGFR-2) with a view to deciphering their possible roles in regeneration.
METHODS
METHODS
In silico analysis of proteins was performed using Clustal omega, Swiss model, MEGA 7.0, etc. Gene expression was studied by whole mount in situ hybridization. VEGF and FGF signaling was inhibited using specific pharmacological inhibitors and their effects on head regeneration were studied.
RESULTS
RESULTS
Expression patterns of the genes indicate a possible interaction between FGF-1 and FGFR-1 and also VEGF and VEGFR-2. Upon treatment of decapitated hydra with pharmacological inhibitor of FGFR-1 or VEGFR-2 for 48 h, head regeneration was delayed in treated as compared to untreated, control regenerates. When we studied the expression of head specific genes HyBra1 and HyKs1 and tentacle specific gene HyAlx in control and treated regenerates using whole mount in situ hybridization, expression of all the three genes was found to be adversely affected in treated regenerates.
CONCLUSIONS
CONCLUSIONS
The results suggest that VEGF and FGF signaling play important roles in regeneration of hypostome and tentacles in hydra.
Identifiants
pubmed: 31421190
pii: S0378-1119(19)30706-1
doi: 10.1016/j.gene.2019.144047
pii:
doi:
Substances chimiques
Indoles
0
Pyrroles
0
SU 5402
0
Vascular Endothelial Growth Factor A
0
Fibroblast Growth Factor 1
104781-85-3
Fibroblast Growth Factors
62031-54-3
Semaxinib
71IA9S35AJ
Receptor, Fibroblast Growth Factor, Type 1
EC 2.7.10.1
Vascular Endothelial Growth Factor Receptor-2
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
144047Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.