Differentiation of live and dead Mycobacterium tuberculosis complex in meat samples using PMA qPCR.
Animals
Animals, Wild
/ microbiology
Cattle
Colony Count, Microbial
DNA, Bacterial
Humans
Limit of Detection
Meat
/ microbiology
Microbial Viability
Mycobacterium tuberculosis
/ isolation & purification
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Tuberculosis, Bovine
/ microbiology
MTC
PMA dye
Venison
bTB
qPCR
Journal
Food microbiology
ISSN: 1095-9998
Titre abrégé: Food Microbiol
Pays: England
ID NLM: 8601127
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
07
01
2019
revised:
15
07
2019
accepted:
16
07
2019
entrez:
19
8
2019
pubmed:
20
8
2019
medline:
18
12
2019
Statut:
ppublish
Résumé
The causative agents of zoonotic bovine tuberculosis (bTB), Mycobacterium bovis and M. caprae, are members of the M. tuberculosis complex (MTC). Wildlife such as red deer infected with bTB are often without pathological findings, thus meat thereof may be classified as safe for human consumption. The culturing of MTC is time consuming and inappropriate to be applied with fresh meat and food. Therefore, a rapid method "PMA qPCR" to differentiate living and dead cells of MTC was developed in this study. By treating with 50 μM PMA™ dye, dead M. bovis BCG (≤10
Identifiants
pubmed: 31421753
pii: S0740-0020(19)30028-0
doi: 10.1016/j.fm.2019.103275
pii:
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103275Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.