Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions.

Crystallography, X-Ray Green Fluorescent Proteins / chemistry HeLa Cells Humans Hydrogen-Ion Concentration Models, Molecular Photochemical Processes Tumor Cells, Cultured
PALM acid tolerance fluorescent proteins pH reversible photoswitching super-resolution fluorescence imaging trans-cis isomerization

Journal

Cell chemical biology
ISSN: 2451-9448
Titre abrégé: Cell Chem Biol
Pays: United States
ID NLM: 101676030

Informations de publication

Date de publication:
17 10 2019
Historique:
received: 03 02 2019
revised: 13 06 2019
accepted: 23 07 2019
pubmed: 20 8 2019
medline: 2 10 2020
entrez: 20 8 2019
Statut: ppublish

Résumé

Reversibly switchable fluorescent proteins (RSFPs) are crucial tags for super-resolution observation of protein localization and dynamics inside living cells. However, due to the high fluorescence pK

Identifiants

DOI: 10.1016/j.chembiol.2019.07.012 PMID: 31422907
pubmed: 31422907
pii: S2451-9456(19)30243-0
doi: 10.1016/j.chembiol.2019.07.012
pii:
doi:

Substances chimiques

Green Fluorescent Proteins 147336-22-9

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1469-1479.e6

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Hajime Shinoda (H)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.

Kai Lu (K)

The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.

Ryosuke Nakashima (R)

The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.

Tetsuichi Wazawa (T)

The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.

Kosuke Noguchi (K)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.

Tomoki Matsuda (T)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan; The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.

Takeharu Nagai (T)

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan; The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan. Electronic address: ng1@sanken.osaka-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Acid-Tolerant Reversibly Switchable Green...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › Acid-Tolerant Reversibly Switchable Green...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Acid-Tolerant Reversibly Switchable Green...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Acid-Tolerant Reversibly Switchable Green...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Acid-Tolerant Reversibly Switchable Green...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Acid-Tolerant Reversibly Switchable Green...
questionsmedicales.fr › Phénomènes chimiques › Processus photochimiques › Acid-Tolerant Reversibly Switchable Green...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Acid-Tolerant Reversibly Switchable Green...