Drosophila Snazarus Regulates a Lipid Droplet Population at Plasma Membrane-Droplet Contacts in Adipocytes.
Drosophila fat body
ER
ER-PM contact site
FA
FB
LD
PM
endoplasmic reticulum
fatty acid
lipid droplet
plasma membrane
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
09 09 2019
09 09 2019
Historique:
received:
25
09
2018
revised:
22
04
2019
accepted:
17
07
2019
pubmed:
20
8
2019
medline:
27
5
2020
entrez:
20
8
2019
Statut:
ppublish
Résumé
Adipocytes store nutrients as lipid droplets (LDs), but how they organize their LD stores to balance lipid uptake, storage, and mobilization remains poorly understood. Here, using Drosophila fat body (FB) adipocytes, we characterize spatially distinct LD populations that are maintained by different lipid pools. We identify peripheral LDs (pLDs) that make close contact with the plasma membrane (PM) and are maintained by lipophorin-dependent lipid trafficking. pLDs are distinct from larger cytoplasmic medial LDs (mLDs), which are maintained by FASN1-dependent de novo lipogenesis. We find that sorting nexin CG1514 or Snazarus (Snz) associates with pLDs and regulates LD homeostasis at ER-PM contact sites. Loss of SNZ perturbs pLD organization, whereas Snz over-expression drives LD expansion, triacylglyceride production, starvation resistance, and lifespan extension through a DESAT1-dependent pathway. We propose that Drosophila adipocytes maintain spatially distinct LD populations and identify Snz as a regulator of LD organization and inter-organelle crosstalk.
Identifiants
pubmed: 31422916
pii: S1534-5807(19)30627-6
doi: 10.1016/j.devcel.2019.07.021
pmc: PMC7446143
mid: NIHMS1600783
pii:
doi:
Substances chimiques
Drosophila Proteins
0
Snz protein, Drosophila
0
Sorting Nexins
0
Fatty Acid Desaturases
EC 1.14.19.-
desat1 protein, Drosophila
EC 1.14.99.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
557-572.e5Subventions
Organisme : NHLBI NIH HHS
ID : P01 HL020948
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM119768
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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