Separation-Based Enzymomics Assay for the Discovery of Altered Peptide-Metabolizing Enzymatic Activities in Biosamples.

Amino Acid Sequence Chromatography, Liquid Colorectal Neoplasms / enzymology Electrophoresis Humans Metalloendopeptidases / analysis Neurotensin / chemistry Peptide Library Proteome / analysis Proteomics / methods Tandem Mass Spectrometry

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
17 09 2019
Historique:
pubmed: 20 8 2019
medline: 29 9 2020
entrez: 20 8 2019
Statut: ppublish

Résumé

We have developed a novel method to globally monitor the enzymatic activities of biological samples based on performing the global activity analysis on a proteome separated by native electrophoresis. The study of the alteration in peptide-metabolizing enzymatic activity in colorectal tumor specimens led us to the discovery of elevated thimet oligopeptidase activity, which contributed to the faster consumption of immune-stimulating peptide neurotensin.

Identifiants

DOI: 10.1021/acs.analchem.9b03016 PMID: 31424921
pubmed: 31424921
doi: 10.1021/acs.analchem.9b03016
doi:

Substances chimiques

Peptide Library 0
Proteome 0
Neurotensin 39379-15-2
Metalloendopeptidases EC 3.4.24.-
thimet oligopeptidase EC 3.4.24.15
neurolysin EC 3.4.24.16

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11497-11501

Auteurs

Yuki Ichihashi (Y)

Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.

Toru Komatsu (T)

Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
ORCID: 0000-0002-9268-6964

Etsu Kyo (E)

Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.

Hiroyuki Matsuzaki (H)

Department of Surgical Oncology, Graduate School of Medicine , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
ORCID: 0000-0001-8521-3029

Keisuke Hata (K)

Department of Surgical Oncology, Graduate School of Medicine , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.

Toshiaki Watanabe (T)

Department of Surgical Oncology, Graduate School of Medicine , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.

Tasuku Ueno (T)

Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.

Kenjiro Hanaoka (K)

Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
ORCID: 0000-0003-0797-4038

Yasuteru Urano (Y)

Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
Graduate School of Medicine , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
Core Research for Evolutional Science and Technology (CREST) Investigator , Japan Agency for Medical Research and Development (AMED) , 1-7-1 Otemachi , Chiyoda-ku , Tokyo 100-0004 , Japan.
ORCID: 0000-0002-1220-6327

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies gastro-intestinales › Maladies intestinales › Maladies du rectum › Tumeurs colorectales › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Metalloproteases › Metalloendopeptidases › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Neuropeptides › Neurotensine › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Banque de gènes › Banque de peptides › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéome › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Separation-Based Enzymomics Assay for the...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Separation-Based Enzymomics Assay for the...