Factors affecting sedimentational separation of bacteria from blood.
E. coli
bacterial bloodstream infection
bacterial separation
centrifugation
disk design
human blood
sedimentation
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
14
01
2019
revised:
19
06
2019
accepted:
01
08
2019
pubmed:
20
8
2019
medline:
9
6
2021
entrez:
20
8
2019
Statut:
ppublish
Résumé
Rapid diagnosis of blood infections requires fast and efficient separation of bacteria from blood. We have developed spinning hollow disks that separate bacteria from blood cells via the differences in sedimentation velocities of these particles. Factors affecting separation included the spinning speed and duration, and disk size. These factors were varied in dozens of experiments for which the volume of separated plasma, and the concentration of bacteria and red blood cells (RBCs) in separated plasma were measured. Data were correlated by a parameter of characteristic sedimentation length, which is the distance that an idealized RBC would travel during the entire spin. Results show that characteristic sedimentation length of 20 to 25 mm produces an optimal separation and collection of bacteria in plasma. This corresponds to spinning a 12-cm-diameter disk at 3,000 rpm for 13 s. Following the spin, a careful deceleration preserves the separation of cells from plasma and provides a bacterial recovery of about 61 ± 5%.
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2892Subventions
Organisme : NIAID NIH HHS
ID : R01 AI116989
Pays : United States
Informations de copyright
© 2019 American Institute of Chemical Engineers.
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