Development, Implementation and Assessment of Molecular Diagnostics by Next Generation Sequencing in Personalized Treatment of Cancer: Experience of a Public Reference Healthcare Hospital.
UNE-EN ISO 15189 accreditation
metastatic colorectal cancer
molecular diagnostics
next generation sequencing
non-small cell lung cancer
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
16 Aug 2019
16 Aug 2019
Historique:
received:
30
07
2019
revised:
12
08
2019
accepted:
13
08
2019
entrez:
21
8
2019
pubmed:
21
8
2019
medline:
21
8
2019
Statut:
epublish
Résumé
The establishment of precision medicine in cancer patients requires the study of several biomarkers. Single-gene testing approaches are limited by sample availability and turnaround time. Next generation sequencing (NGS) provides an alternative for detecting genetic alterations in several genes with low sample requirements. Here we show the implementation to routine diagnostics of a NGS assay under International Organization for Standardization (UNE-EN ISO 15189:2013) accreditation. For this purpose, 106 non-small cell lung cancer (NSCLC) and 102 metastatic colorectal cancer (mCRC) specimens were selected for NGS analysis with Oncomine Solid Tumor (ThermoFisher). In NSCLC the most prevalently mutated gene was
Identifiants
pubmed: 31426418
pii: cancers11081196
doi: 10.3390/cancers11081196
pmc: PMC6721584
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministerio de Economía, Industria y Competitividad, Programa Estatal de I+D+i Orientada a los Retos de la Sociedad, Convocatoria Retos-Colaboración 2015
ID : RTC-2015-3625-1
Organisme : Roche Diagnostics
ID : IISLaFe 2017/0070
Organisme : Consellería de Educación, Investigación, Cultura y Deporte de la Comunidad Valenciana
ID : ACIF/2018/258
Déclaration de conflit d'intérêts
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
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