The TRAPP complex mediates secretion arrest induced by stress granule assembly.
COPII
Cdk
TRAPP complex
integrated stress response
stress granules
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
01 10 2019
01 10 2019
Historique:
received:
04
02
2019
revised:
31
07
2019
accepted:
01
08
2019
pubmed:
21
8
2019
medline:
31
12
2019
entrez:
21
8
2019
Statut:
ppublish
Résumé
The TRAnsport Protein Particle (TRAPP) complex controls multiple membrane trafficking steps and is strategically positioned to mediate cell adaptation to diverse environmental conditions, including acute stress. We have identified the TRAPP complex as a component of a branch of the integrated stress response that impinges on the early secretory pathway. The TRAPP complex associates with and drives the recruitment of the COPII coat to stress granules (SGs) leading to vesiculation of the Golgi complex and arrest of ER export. The relocation of the TRAPP complex and COPII to SGs only occurs in cycling cells and is CDK1/2-dependent, being driven by the interaction of TRAPP with hnRNPK, a CDK substrate that associates with SGs when phosphorylated. In addition, CDK1/2 inhibition impairs TRAPP complex/COPII relocation to SGs while stabilizing them at ER exit sites. Importantly, the TRAPP complex controls the maturation of SGs. SGs that assemble in TRAPP-depleted cells are smaller and are no longer able to recruit RACK1 and Raptor, two TRAPP-interactive signaling proteins, sensitizing cells to stress-induced apoptosis.
Identifiants
pubmed: 31429971
doi: 10.15252/embj.2019101704
pmc: PMC6769382
doi:
Substances chimiques
Membrane Transport Proteins
0
CDC2 Protein Kinase
EC 2.7.11.22
Cyclin-Dependent Kinase 2
EC 2.7.11.22
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e101704Subventions
Organisme : EC | H2020 | H2020 Priority Excellent Science | H2020 European Research Council (ERC)
ID : 670881
Pays : International
Organisme : Associazione Italiana per la Ricerca sul Cancro (AIRC)
ID : IG2013_14761
Pays : International
Organisme : Telethon
ID : TGM11CB1
Pays : Italy
Informations de copyright
© 2019 The Authors.
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