A kinome-wide screen using a NanoLuc LATS luminescent biosensor identifies ALK as a novel regulator of the Hippo pathway in tumorigenesis and immune evasion.
Adaptor Proteins, Signal Transducing
/ genetics
Anaplastic Lymphoma Kinase
/ genetics
Animals
Biosensing Techniques
Breast Neoplasms
/ genetics
Carcinogenesis
/ genetics
Female
HEK293 Cells
Hippo Signaling Pathway
Humans
Lung Neoplasms
/ genetics
Mice
Mice, Knockout
Protein Serine-Threonine Kinases
/ genetics
Signal Transduction
/ genetics
Transcription Factors
/ genetics
Tumor Escape
YAP-Signaling Proteins
PD-L1
breast cancer
immunotherapy
kinases
lung cancer
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
pubmed:
23
8
2019
medline:
27
5
2020
entrez:
22
8
2019
Statut:
ppublish
Résumé
The Hippo pathway is an emerging signaling pathway that plays important roles in organ size control, tissue homeostasis, tumorigenesis, metastasis, drug resistance, and immune response. Although many regulators of the Hippo pathway have been reported, the extracellular stimuli and kinase regulators of the Hippo pathway remain largely unknown. To identify novel regulars of the Hippo pathway, in this study we created the first ultra-bright NanoLuc biosensor (BS) to monitor the activity of large tumor suppressor (LATS) kinase 1, a central player of the Hippo pathway. We show that this NanoLuc BS achieves significantly advanced sensitivity and stability both
Identifiants
pubmed: 31431076
doi: 10.1096/fj.201901343R
pmc: PMC6902680
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Transcription Factors
0
YAP-Signaling Proteins
0
YAP1 protein, human
0
ALK protein, human
EC 2.7.10.1
Anaplastic Lymphoma Kinase
EC 2.7.10.1
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12487-12499Subventions
Organisme : Canadian Institute of Health Research
ID : 119325
Pays : International
Organisme : Canadian Institute of Health Research
ID : 148629
Pays : International
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