Mitochondrial dysfunction increases fatty acid β-oxidation and translates into impaired neuroblast maturation.
Neuro2a cells
energy metabolism
mitochondria
neuron maturation
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
14
06
2019
revised:
25
07
2019
accepted:
11
08
2019
pubmed:
23
8
2019
medline:
23
6
2020
entrez:
22
8
2019
Statut:
ppublish
Résumé
The metabolic transition from anaerobic glycolysis and fatty acid β-oxidation to glycolysis coupled to oxidative phosphorylation is a key process for the transition of quiescent neural stem cells to proliferative neural progenitor cells. However, a full characterization of the metabolic shift and the involvement of mitochondria during the last step of neurogenesis, from neuroblasts to neuron maturation, is still elusive. Here, we describe a model of neuroblasts, Neuro2a cells, with impaired differentiation capacity due to mitochondrial dysfunction. Using a detailed biochemical characterization consisting of steady-state metabolomics and metabolic flux analysis, we find increased fatty acid β-oxidation as a peculiar feature of neuroblasts with altered mitochondria. The consequent metabolic switch favors neuroblast proliferation at the expense of neuron maturation.
Identifiants
pubmed: 31432511
doi: 10.1002/1873-3468.13584
doi:
Substances chimiques
Fatty Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3173-3189Subventions
Organisme : Università degli Studi di Milano
Pays : International
Organisme : Cariplo Foundation
ID : 2014-0991
Pays : International
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca Progetto di Eccellenza
Pays : International
Organisme : Giovanni Armenise Harvard-Foundation Career Development Grant
Pays : International
Informations de copyright
© 2019 Federation of European Biochemical Societies.
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