Invasion of human microvascular endothelial cells by Mycobacterium leprae through Mce1A protein.
Mycobacterium leprae
Hansen's disease
endothelial cell
mammalian cell entry protein
mce1A gene
Journal
The Journal of dermatology
ISSN: 1346-8138
Titre abrégé: J Dermatol
Pays: England
ID NLM: 7600545
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
24
04
2019
accepted:
22
07
2019
pubmed:
23
8
2019
medline:
14
11
2019
entrez:
22
8
2019
Statut:
ppublish
Résumé
In patients with lepromatous leprosy, Mycobacterium leprae is often observed inside the human microvascular endothelial cells (HMVEC) surrounding Schwann cells (SC) at the site of lesions in the peripheral nerves. Based on this observation, it is considered that the nasal mucous may be the invasion pathway for M. leprae and HMVEC serve as an important reservoir for the bacteria before they invade SC. In light of previous research which revealed that Mce1A protein mediates bacterial invasion into nasal epithelial cells and HMVEC, we conducted a study to determine whether the invasion of M. leprae into HMVEC can be suppressed by blocking the Mce1A protein. In this study, we analyzed bacterial invasive activity by adding recombinant Escherichia coli, which express the active region (InvX:72 a.a.) of Mce1A protein on their external membrane, into cultured HMVEC, using the adhesin involved in the diffuse adherence mechanism. The number of bacteria that invaded into the cells was then measured by a colony counting method. The active region of Mce1A was divided into four sections, and hyperimmune antisera was prepared for each section for analyzing the inhibitory effect against invasion. The invasive activity was suppressed by antibodies against InvX regions 1-24 a.a., 25-46 a.a. and 58-72 a.a. This suggests that the InvX regions 1-24 a.a., 25-46 a.a. and 58-72 a.a. of Mce1A protein play an important role in the invasion of M. leprae into HMVEC and that it may be possible to suppress entry of M. leprae in HMVEC with antibodies against these regions.
Identifiants
pubmed: 31432529
doi: 10.1111/1346-8138.15047
doi:
Substances chimiques
Antibodies, Bacterial
0
Bacterial Proteins
0
Immune Sera
0
Mce1A protein, Mycobacterium leprae
0
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
853-858Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 Japanese Dermatological Association.
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