A Passive Microfluidic Device for Chemotaxis Studies.
chemotaxis
diffusion
mini incubator
passive microfluidic device
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
20 Aug 2019
20 Aug 2019
Historique:
received:
10
07
2019
revised:
11
08
2019
accepted:
16
08
2019
entrez:
23
8
2019
pubmed:
23
8
2019
medline:
23
8
2019
Statut:
epublish
Résumé
This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO
Identifiants
pubmed: 31434220
pii: mi10080551
doi: 10.3390/mi10080551
pmc: PMC6722731
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministero della Salute
ID : (CUP J65C13001350001, project No. GR-2010-2311677
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