Force-induced conformational changes in PIEZO1.
Journal
Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
14
11
2018
accepted:
25
07
2019
pubmed:
23
8
2019
medline:
27
3
2020
entrez:
23
8
2019
Statut:
ppublish
Résumé
PIEZO1 is a mechanosensitive channel that converts applied force into electrical signals. Partial molecular structures show that PIEZO1 is a bowl-shaped trimer with extended arms. Here we use cryo-electron microscopy to show that PIEZO1 adopts different degrees of curvature in lipid vesicles of different sizes. We also use high-speed atomic force microscopy to analyse the deformability of PIEZO1 under force in membranes on a mica surface, and show that PIEZO1 can be flattened reversibly into the membrane plane. By approximating the absolute force applied, we estimate a range of values for the mechanical spring constant of PIEZO1. Both methods of microscopy demonstrate that PIEZO1 can deform its shape towards a planar structure. This deformation could explain how lateral membrane tension can be converted into a conformation-dependent change in free energy to gate the PIEZO1 channel in response to mechanical perturbations.
Identifiants
pubmed: 31435018
doi: 10.1038/s41586-019-1499-2
pii: 10.1038/s41586-019-1499-2
pmc: PMC7258172
mid: NIHMS1585185
doi:
Substances chimiques
Aluminum Silicates
0
Ion Channels
0
Liposomes
0
Piezo1 protein, mouse
0
mica
V8A1AW0880
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
230-234Subventions
Organisme : NCCIH NIH HHS
ID : DP1 AT010874
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Commentaires et corrections
Type : CommentIn
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