Optimization of extraction of polysaccharide from dandelion root by response surface methodology: Structural characterization and antioxidant activity.
Antioxidant activity
Dandelion root polysaccharides
Response surface methodology
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Nov 2019
01 Nov 2019
Historique:
received:
09
02
2019
revised:
17
08
2019
accepted:
18
08
2019
pubmed:
23
8
2019
medline:
16
4
2020
entrez:
23
8
2019
Statut:
ppublish
Résumé
In this study, response surface methodology (RSM) was used to optimize the extraction parameters of dandelion root polysaccharide (DRP). Two novel polysaccharides (DRP-2b, DRP-3a) were isolated from dandelion root by graded ethanol precipitation and column chromatography. Structural analyses indicated that DRP-2b, with a molecular weight of 31.8 kDa, was composed of rhamnose, glucuronic acid, glucose, galactose and arabinose, whereas DRP-3a, with a molecular weight of 6.72 kDa, was composed of rhamnose, glucose, galactose and arabinose. The backbones of DRP-2b and DRP-3a were mainly composed of (1 → 5)-linked-α-D-Ara and (1 → 6)-linked-α-D-Glc, respectively. In addition, the antioxidant activities and protective effects against H
Identifiants
pubmed: 31437509
pii: S0141-8130(19)31014-1
doi: 10.1016/j.ijbiomac.2019.08.161
pii:
doi:
Substances chimiques
Antioxidants
0
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
907-919Informations de copyright
Copyright © 2019. Published by Elsevier B.V.