Evaluation of immunogenicity of novel multi-epitope subunit vaccines in combination with poly I:C against Brucella melitensis and Brucella abortus infection.
Animals
Antibodies, Bacterial
/ blood
Antigens, Bacterial
/ immunology
Brucella abortus
/ immunology
Brucella melitensis
/ immunology
Brucellosis
/ prevention & control
Cell Proliferation
/ drug effects
Cytokines
/ immunology
Epitopes, B-Lymphocyte
/ immunology
Epitopes, T-Lymphocyte
/ immunology
Female
Immunoglobulin G
/ blood
Lymphocytes
/ drug effects
Mice, Inbred BALB C
Poly I-C
/ administration & dosage
Vaccines, Subunit
/ administration & dosage
Brucella
Immune responses
Multi-epitope vaccine
Poly I:C
Journal
International immunopharmacology
ISSN: 1878-1705
Titre abrégé: Int Immunopharmacol
Pays: Netherlands
ID NLM: 100965259
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
05
07
2019
revised:
12
08
2019
accepted:
12
08
2019
pubmed:
23
8
2019
medline:
14
2
2020
entrez:
23
8
2019
Statut:
ppublish
Résumé
Brucellosis is a worldwide zoonotic disease affecting domestic animals and humans. Due to several safety problems associated with live attenuated vaccines (Rev1 and RB51), it is necessary to produce an efficient and safer vaccine against Brucella. In this study, we evaluated efficacy of two novel multi-peptide vaccine candidates of FliC, 7α-HSDH, BhuA antigens with and without poly I:C adjuvant. Hence, humoral and cellular immune responses and protective efficacy were determined in immunized mice. Our investigation indicated that multi-epitope antigens showed a significant induction of Th1 immunity with high levels of specific IgG (especially the IgG2a), as well as IFN-γ and IL-2 compared to the control group. The addition of poly I:C to multi-epitope antigens improved the humoral and cellular immune responses. The multi-epitope antigens with and without poly I:C also provided cross protection against B. melitensis16M and B. abortus544 infections. The present study suggests that the novel multi-epitope vaccine candidates based on B cell, CD4
Identifiants
pubmed: 31437796
pii: S1567-5769(19)31475-4
doi: 10.1016/j.intimp.2019.105829
pii:
doi:
Substances chimiques
Antibodies, Bacterial
0
Antigens, Bacterial
0
Cytokines
0
Epitopes, B-Lymphocyte
0
Epitopes, T-Lymphocyte
0
Immunoglobulin G
0
Vaccines, Subunit
0
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105829Informations de copyright
Copyright © 2019. Published by Elsevier B.V.