A new method for quantifying causative and diagnostic markers of methylenecyclopropylglycine poisoning.
AM, atypical myopathy
Ackee fruit
Acquired multiple acyl-CoA dehydrogenase deficiency
HGA, hypoglycin A
Hypoglycin A (PubChem CID: 9081)
Litchi
MCPA-G, methylenecyclopropylacetyl glycine
MCPF, methylenecyclopropylformate
MCPF-G, methylenecyclopropylformyl glycine
MCPG, methylenecyclopropylglycine
Methylenecyclopropylacetyl glycine (PubChem: 6429414)
Methylenecyclopropylformate
Methylenecyclopropylformyl glycine (PubChem: 129713646)
Methylenecyclopropylglycine
Methylenecyclopropylglycine (PubChem CID: 142776)
Sapindaceae
Journal
Toxicology reports
ISSN: 2214-7500
Titre abrégé: Toxicol Rep
Pays: Ireland
ID NLM: 101630272
Informations de publication
Date de publication:
2019
2019
Historique:
received:
28
03
2019
revised:
29
07
2019
accepted:
02
08
2019
entrez:
24
8
2019
pubmed:
24
8
2019
medline:
24
8
2019
Statut:
epublish
Résumé
Up to now quantification of hypoglycin A in serum and urine in the range of nmols to μmols per liter plus the measurement of accumulated acyl conjugates have been used for the diagnosis of poisoning by fruits or seeds of Methylenecyclopropylglycine was extracted from serum and urine of a volunteer by a methanolic solution containing labeled methylenecyclopropylglycine as internal standard. UPLC-MS/MS analysis was performed after butylation. Lower limits of detection and quantification were found at 0.5 and 2.5 nmol/L respectively in both urine and serum for each of two isomers, linearity of results (r Quantification of methylenecyclopropylglycine can be successfully integrated into our established analytical procedure used for clinical diagnosis of
Sections du résumé
BACKGROUND
BACKGROUND
Up to now quantification of hypoglycin A in serum and urine in the range of nmols to μmols per liter plus the measurement of accumulated acyl conjugates have been used for the diagnosis of poisoning by fruits or seeds of
METHOD
METHODS
Methylenecyclopropylglycine was extracted from serum and urine of a volunteer by a methanolic solution containing labeled methylenecyclopropylglycine as internal standard. UPLC-MS/MS analysis was performed after butylation.
RESULTS
RESULTS
Lower limits of detection and quantification were found at 0.5 and 2.5 nmol/L respectively in both urine and serum for each of two isomers, linearity of results (r
CONCLUSIONS
CONCLUSIONS
Quantification of methylenecyclopropylglycine can be successfully integrated into our established analytical procedure used for clinical diagnosis of
Identifiants
pubmed: 31440457
doi: 10.1016/j.toxrep.2019.08.002
pii: S2214-7500(19)30185-4
pmc: PMC6699453
doi:
Types de publication
Journal Article
Langues
eng
Pagination
803-808Déclaration de conflit d'intérêts
None.
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