Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue.
Adipose Tissue
/ cytology
Animals
Cell Differentiation
/ drug effects
Cells, Cultured
Diffusion Tensor Imaging
/ methods
Fibroblasts
/ pathology
Humans
Ischemia
/ pathology
Male
Mesenchymal Stem Cells
/ cytology
Mice, Transgenic
Neovascularization, Physiologic
/ physiology
Regeneration
/ drug effects
diffusion
ischaemia
magnetic resonance imaging
mesenchymal stromal cells
peripheral artery disease
transplantation
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
10
04
2019
revised:
25
06
2019
accepted:
09
07
2019
pubmed:
24
8
2019
medline:
21
12
2019
entrez:
24
8
2019
Statut:
ppublish
Résumé
Application of non-invasive imaging methods plays an important role in the assessment of cellular therapy effects in peripheral artery disease. The purpose of this work was to evaluate the kinetics of MRI-derived parameters characterizing ischaemic hindlimb muscle after administration of human mesenchymal stromal cells derived from adipose tissue (hADSC) in mice. MRI experiments were performed on a 9.4T Bruker system. The measurement protocol included transverse relaxation time mapping and diffusion tensor imaging. The monitoring period encompassed 14 days after femoral artery ligation and subsequent cell administration. The effect of hADSC transplantation was compared with the effect of normal human dermal fibroblasts (NHDFs) and phosphate-buffered saline injection. The most significant differences between the hADSC group and the remaining ones were observed around day 3 after ischaemia induction (increased transverse relaxation time in the hADSC group in comparison with the control group) and around day 7 (increased transverse relaxation time and decreased third eigenvalue of the diffusion tensor in the hADSC group in comparison with the control and NHDF groups) at the site of hADSC injection. Histologically, it was associated with increased macrophage infiltration at days 3-7 and with the presence of small regenerating fibres in the ischaemic tissue at day 7. Our results underscore the important role of macrophages in mediating the therapeutic effects of hADSCs and confirm the huge potential of magnetic resonance imaging in monitoring of cellular therapy effects.
Identifiants
pubmed: 31441162
doi: 10.1111/cpr.12672
pmc: PMC6869084
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12672Subventions
Organisme : National Science Centre Poland
ID : UMO-2014/15/B/NZ4/00696
Informations de copyright
© 2019 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
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