Early Diagnosis of Pathogen Infection by Cell-Based Activation Immunoassay.
Bacillus anthracis
ELISPOT
Early detection
Francisella tularensis
Influenza virus
Yersinia pestis
animal models
infection biomarkers
lymphocyte activation
lymphocyte transcriptomics
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
22 08 2019
22 08 2019
Historique:
received:
05
08
2019
revised:
14
08
2019
accepted:
21
08
2019
entrez:
25
8
2019
pubmed:
25
8
2019
medline:
19
5
2020
Statut:
epublish
Résumé
Diagnostic identification of pathogens is usually accomplished by isolation of the pathogen or its substances, and should correlate with the time and site of infection. Alternatively, immunoassays such as enzyme-linked immunosorbent assay (ELISA) tests for quantification of serum antibodies are expedient and are usually employed for retrospective diagnostic of a particular infective agent. Here, the potential of cell-based immunoassays for early pathogen detection was evaluated by quantification of specific, antigen-activated, low-frequency IFNγ-secreting cells in mouse spleens following infection with various pathogens. Using enzyme-linked immunospot (ELISPOT) assays, specific responses were observed within 3-6 days following infection with
Identifiants
pubmed: 31443439
pii: cells8090952
doi: 10.3390/cells8090952
pmc: PMC6769711
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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