Molecular organization of mammalian meiotic chromosome axis revealed by expansion STORM microscopy.
STORM
chromosome axis
expansion microscopy
meiosis
synaptonemal complex
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
10 09 2019
10 09 2019
Historique:
pubmed:
25
8
2019
medline:
4
4
2020
entrez:
25
8
2019
Statut:
ppublish
Résumé
During prophase I of meiosis, chromosomes become organized as loop arrays around the proteinaceous chromosome axis. As homologous chromosomes physically pair and recombine, the chromosome axis is integrated into the tripartite synaptonemal complex (SC) as this structure's lateral elements (LEs). While the components of the mammalian chromosome axis/LE-including meiosis-specific cohesin complexes, the axial element proteins SYCP3 and SYCP2, and the HORMA domain proteins HORMAD1 and HORMAD2-are known, the molecular organization of these components within the axis is poorly understood. Here, using expansion microscopy coupled with 2-color stochastic optical reconstruction microscopy (STORM) imaging (ExSTORM), we address these issues in mouse spermatocytes at a resolution of 10 to 20 nm. Our data show that SYCP3 and the SYCP2 C terminus, which are known to form filaments in vitro, form a compact core around which cohesin complexes, HORMADs, and the N terminus of SYCP2 are arrayed. Overall, our study provides a detailed structural view of the meiotic chromosome axis, a key organizational and regulatory component of meiotic chromosomes.
Identifiants
pubmed: 31444302
pii: 1902440116
doi: 10.1073/pnas.1902440116
pmc: PMC6744910
doi:
Substances chimiques
Cell Cycle Proteins
0
DNA-Binding Proteins
0
HORMAD2 protein, mouse
0
Nohma protein, mouse
0
Sycp2 protein, mouse
0
Sycp3 protein, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
18423-18428Subventions
Organisme : NIBIB NIH HHS
ID : DP2 EB020400
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI082850
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM104141
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Mol Cell. 2000 Jan;5(1):73-83
pubmed: 10678170
Annu Rev Genet. 1999;33:603-754
pubmed: 10690419
J Microsc. 2000 May;198(Pt 2):82-7
pubmed: 10810003
Science. 2002 May 10;296(5570):1115-8
pubmed: 12004129
Mol Biol Cell. 2004 Feb;15(2):827-37
pubmed: 14657244
Nat Cell Biol. 2004 Jun;6(6):555-62
pubmed: 15146193
Dev Cell. 2005 Jun;8(6):949-61
pubmed: 15935783
Nat Genet. 2005 Dec;37(12):1351-5
pubmed: 16258540
J Cell Biol. 2006 May 22;173(4):497-507
pubmed: 16717126
Nat Methods. 2006 Oct;3(10):793-5
pubmed: 16896339
Science. 2006 Sep 15;313(5793):1642-5
pubmed: 16902090
Annu Rev Cell Dev Biol. 2008;24:105-29
pubmed: 18616427
Methods Mol Biol. 2009;558:355-82
pubmed: 19685335
PLoS Genet. 2009 Oct;5(10):e1000702
pubmed: 19851446
PLoS Genet. 2010 Aug 12;6(8):null
pubmed: 20711356
PLoS Genet. 2010 Nov 04;6(11):e1001190
pubmed: 21079677
EMBO J. 2011 Jul 08;30(15):3091-105
pubmed: 21743440
Nat Methods. 2013 Jun;10(6):557-62
pubmed: 23624665
Nat Rev Genet. 2013 Nov;14(11):794-806
pubmed: 24136506
Genes Dev. 2014 Mar 15;28(6):594-607
pubmed: 24589552
Elife. 2014 Jun 20;3:null
pubmed: 24950965
PLoS Genet. 2014 Jul 03;10(7):e1004413
pubmed: 24992337
Nat Struct Mol Biol. 2014 Oct;21(10):864-70
pubmed: 25173175
Science. 2014 Nov 21;346(6212):963-7
pubmed: 25414305
Dev Cell. 2014 Nov 24;31(4):487-502
pubmed: 25446517
Science. 2015 Jan 30;347(6221):543-8
pubmed: 25592419
Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):2029-33
pubmed: 25646409
Elife. 2015 Aug 10;4:null
pubmed: 26258962
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14635-40
pubmed: 26561583
EMBO J. 2016 Apr 1;35(7):759-72
pubmed: 26896444
Nat Methods. 2016 Jun;13(6):485-8
pubmed: 27064647
Exp Cell Res. 2016 May 15;344(1):22-29
pubmed: 27090018
Nat Struct Mol Biol. 2016 May 4;23(5):369-77
pubmed: 27142324
G3 (Bethesda). 2016 Jun 01;6(6):1713-24
pubmed: 27172213
Nat Biotechnol. 2016 Sep;34(9):987-92
pubmed: 27376584
Protein Cell. 2017 Jul;8(7):538-543
pubmed: 28150150
Nat Methods. 2017 Jun;14(6):593-599
pubmed: 28417997
Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):E4734-E4743
pubmed: 28559338
Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):E6857-E6866
pubmed: 28760978
ACS Nano. 2017 Dec 26;11(12):12677-12686
pubmed: 29165993
Nucleic Acids Res. 2018 Jan 9;46(1):279-292
pubmed: 29186573
Kidney Int. 2018 Apr;93(4):1008-1013
pubmed: 29241621
ACS Nano. 2018 May 22;12(5):4178-4185
pubmed: 29672025
EMBO Rep. 2018 Sep;19(9):
pubmed: 29987134
Elife. 2018 Aug 15;7:
pubmed: 30109982
Nat Methods. 2019 Jan;16(1):71-74
pubmed: 30559430
Science. 2019 Jan 18;363(6424):
pubmed: 30655415
Elife. 2019 Jan 18;8:null
pubmed: 30657449
J Biophotonics. 2019 Aug;12(8):e201900018
pubmed: 30980601
Exp Cell Res. 1996 Jul 10;226(1):20-30
pubmed: 8660935
Nucleic Acids Res. 1998 Jun 1;26(11):2572-9
pubmed: 9592139