An All-Inclusive and Straightway Laboratory Activity to Solve the Three-Dimensional Crystal Structure of a Protein.
Laboratory exercises
X-ray crystallography
computer-based learning
molecular visualization
protein structure function and folding
Journal
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology
ISSN: 1539-3429
Titre abrégé: Biochem Mol Biol Educ
Pays: United States
ID NLM: 100970605
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
16
11
2018
revised:
26
05
2019
accepted:
12
08
2019
pubmed:
25
8
2019
medline:
4
12
2019
entrez:
25
8
2019
Statut:
ppublish
Résumé
X-ray crystallography provides structural information of molecules at the atomic level, being a central technique at the forefront of science and technology. However, crystallography teaching is not usually implemented in biochemistry lab classes due to its complex execution by nonexpert users. Here, we report the basic step-by-step workflow performed by crystallographers in order to solve the three-dimensional structure of a protein. All these activities were executed in a course for Latin-American graduate students with no previous knowledge on X-ray crystallography entitled "Crystallography in Structural Biology: why do we need a protein crystal, and how do we get it?." We would like to share our experience with the educational research community, with the main purpose being to enrich teaching in biochemistry and structural molecular biology by performing a series of interesting laboratory and computer experiments. © 2019 International Union of Biochemistry and Molecular Biology, 47(6):700-707, 2019.
Substances chimiques
hen egg lysozyme
EC 3.2.1.-
Muramidase
EC 3.2.1.17
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
700-707Informations de copyright
© 2019 International Union of Biochemistry and Molecular Biology.
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