Behavioral and molecular markers of death in Drosophila melanogaster.
Aging
/ physiology
Alzheimer Disease
/ physiopathology
Animals
Behavior, Animal
Death
Disease Models, Animal
Drosophila melanogaster
/ physiology
Female
Flight, Animal
/ physiology
Fluorescence
Gravity Sensing
/ physiology
Green Fluorescent Proteins
/ metabolism
Male
Microscopy, Fluorescence
Movement
/ physiology
Optical Imaging
/ methods
Retina
/ metabolism
Video Recording
Alzheimer's disease
Biomarker
Death dance
Death fluorescence
Seizure
Video tracking
Journal
Experimental gerontology
ISSN: 1873-6815
Titre abrégé: Exp Gerontol
Pays: England
ID NLM: 0047061
Informations de publication
Date de publication:
15 10 2019
15 10 2019
Historique:
received:
26
04
2019
revised:
15
07
2019
accepted:
19
08
2019
pubmed:
25
8
2019
medline:
14
7
2020
entrez:
25
8
2019
Statut:
ppublish
Résumé
Fly movement was tracked through 3-dimensional (3D) space as the fly died, using either reflected visible light, reflected infrared (IR) light, or fly GFP fluorescence. Behaviors measured included centrophobism, negative geotaxis, velocity, and total activity. In addition, frequency of directional heading changes (FDHC) was calculated as a measure of erratic movement. Nine middle-aged flies were tracked as they died during normal aging, and fifteen young flies were tracked as they died from dehydration/starvation stress. Episodes of increased FDHC were observed 0-8 h prior to death for the majority of the flies. FDHC was also increased with age in flies with neuronal expression of a human Abeta42 protein fragment associated with Alzheimer's disease. Finally, green autofluorescence appeared in the eye and body immediately prior to and coincident with death, and fluorescence of GFP targeted to the retina increased immediately prior to and coincident with death. The results suggest the potential utility of FDHC, green autofluorescence, and retinal GFP as markers of neuronal malfunction and imminent death.
Identifiants
pubmed: 31445108
pii: S0531-5565(19)30305-5
doi: 10.1016/j.exger.2019.110707
pmc: PMC6744963
mid: NIHMS1538359
pii:
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110707Subventions
Organisme : NIA NIH HHS
ID : R01 AG057741
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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