Neutral Lipopolyplexes for In Vivo Delivery of Conventional and Replicative RNA Vaccine.
mRNA delivery
self-amplifying RNA
splenic dendritic cells
targeting
Journal
Molecular therapy. Nucleic acids
ISSN: 2162-2531
Titre abrégé: Mol Ther Nucleic Acids
Pays: United States
ID NLM: 101581621
Informations de publication
Date de publication:
06 Sep 2019
06 Sep 2019
Historique:
received:
19
02
2019
revised:
17
07
2019
accepted:
17
07
2019
pubmed:
26
8
2019
medline:
26
8
2019
entrez:
26
8
2019
Statut:
ppublish
Résumé
Nucleic acid vaccination relies on injecting DNA or RNA coding antigen(s) to induce a protective immune response. RNA vaccination is being increasingly used in preclinical and clinical studies. However, few delivery systems have been reported for in vivo delivery of RNA of different sizes. Using a tripartite formulation with RNA, cationic polymer, and anionic liposomes, we were able to encapsulate RNA into neutral lipopolyplexes (LPPs). LPPs were stable in vitro and successfully delivered conventional RNA and replicative RNA to dendritic cells in cellulo. Their injection led to reporter gene expression in mice. Finally, administration of LPP-Replicon RNA (RepRNA) led to an adaptive immune response against the antigen coded by the RepRNA. Accordingly, LPPs may represent a universal formulation for RNA delivery.
Identifiants
pubmed: 31446119
pii: S2162-2531(19)30204-5
doi: 10.1016/j.omtn.2019.07.014
pmc: PMC6716064
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
767-775Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
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