Characterization of DLL3-positive circulating tumor cells (CTCs) in patients with small cell lung cancer (SCLC) and evaluation of their clinical relevance during front-line treatment.
Antineoplastic Combined Chemotherapy Protocols
/ adverse effects
Biomarkers
Biomarkers, Tumor
Cell Line, Tumor
Disease Management
Female
Fluorescent Antibody Technique
Gene Expression
Humans
Immunophenotyping
Intracellular Signaling Peptides and Proteins
/ genetics
Leukocytes, Mononuclear
Lung Neoplasms
/ diagnosis
Male
Membrane Proteins
/ genetics
Neoplasm Staging
Neoplastic Cells, Circulating
/ metabolism
Proportional Hazards Models
Small Cell Lung Carcinoma
/ diagnosis
Vimentin
/ genetics
CTCs
DLL3
SCLC
Vimentin
Journal
Lung cancer (Amsterdam, Netherlands)
ISSN: 1872-8332
Titre abrégé: Lung Cancer
Pays: Ireland
ID NLM: 8800805
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
24
03
2019
revised:
11
06
2019
accepted:
26
06
2019
entrez:
27
8
2019
pubmed:
27
8
2019
medline:
14
7
2020
Statut:
ppublish
Résumé
The aim of the study was to characterize and evaluate the presence of DLL3-positive Circulating Tumor Cells (CTCs) in SCLC patients receiving front-line chemotherapy and assess their clinical relevance. Peripheral blood was obtained from treatment-naïve patients with SCLC (n = 108 patients), after one etoposide/platinum cycle (n = 68 patients) and on disease progression (n = 48 patients). Immunofluorescence staining using antibodies against the DLL3, cytokeratins (CK), CD45 and vimentin (Vim) was used for the detection and characterization of CTCs. Before treatment, 74.1% of patients had detectable DLL3 These findings demonstrate an important heterogeneity of CTCs, based on the expression of CK, Vim and DLL3, in patients with SCLC and the changes of DLL3
Identifiants
pubmed: 31447000
pii: S0169-5002(19)30518-5
doi: 10.1016/j.lungcan.2019.06.025
pii:
doi:
Substances chimiques
Biomarkers
0
Biomarkers, Tumor
0
DLL3 protein, human
0
Intracellular Signaling Peptides and Proteins
0
Membrane Proteins
0
Vimentin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-39Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.