A new paradigm for lung-conservative total liquid ventilation.


Journal

EBioMedicine
ISSN: 2352-3964
Titre abrégé: EBioMedicine
Pays: Netherlands
ID NLM: 101647039

Informations de publication

Date de publication:
Feb 2020
Historique:
received: 09 07 2019
revised: 06 08 2019
accepted: 12 08 2019
pubmed: 27 8 2019
medline: 9 10 2020
entrez: 27 8 2019
Statut: ppublish

Résumé

Total liquid ventilation (TLV) of the lungs could provide radically new benefits in critically ill patients requiring lung lavage or ultra-fast cooling after cardiac arrest. It consists in an initial filling of the lungs with perfluorocarbons and subsequent tidal ventilation using a dedicated liquid ventilator. Here, we propose a new paradigm for a lung-conservative TLV using pulmonary volumes of perfluorocarbons below functional residual capacity (FRC). Using a dedicated technology, we showed that perfluorocarbon end-expiratory volumes could be maintained below expected FRC and lead to better respiratory recovery, preserved lung structure and accelerated evaporation of liquid residues as compared to complete lung filling in piglets. Such TLV below FRC prevented volutrauma through preservation of alveolar recruitment reserve. When used with temperature-controlled perfluorocarbons, this lung-conservative approach provided neuroprotective ultra-fast cooling in a model of hypoxic-ischemic encephalopathy. The scale-up and automating of the technology confirmed that incomplete initial lung filling during TLV was beneficial in human adult-sized pigs, despite larger size and maturity of the lungs. Our results were confirmed in aged non-human primates, confirming the safety of this lung-conservative approach. This study demonstrated that TLV with an accurate control of perfluorocarbon volume below FRC could provide the full potential of TLV in an innovative and safe manner. This constitutes a new paradigm through the tidal liquid ventilation of incompletely filled lungs, which strongly differs from the previously known TLV approach, opening promising perspectives for a safer clinical translation. FUND: ANR (COOLIVENT), FRM (DBS20140930781), SATT IdfInnov (project 273).

Sections du résumé

BACKGROUND BACKGROUND
Total liquid ventilation (TLV) of the lungs could provide radically new benefits in critically ill patients requiring lung lavage or ultra-fast cooling after cardiac arrest. It consists in an initial filling of the lungs with perfluorocarbons and subsequent tidal ventilation using a dedicated liquid ventilator. Here, we propose a new paradigm for a lung-conservative TLV using pulmonary volumes of perfluorocarbons below functional residual capacity (FRC).
METHODS AND FINDINGS RESULTS
Using a dedicated technology, we showed that perfluorocarbon end-expiratory volumes could be maintained below expected FRC and lead to better respiratory recovery, preserved lung structure and accelerated evaporation of liquid residues as compared to complete lung filling in piglets. Such TLV below FRC prevented volutrauma through preservation of alveolar recruitment reserve. When used with temperature-controlled perfluorocarbons, this lung-conservative approach provided neuroprotective ultra-fast cooling in a model of hypoxic-ischemic encephalopathy. The scale-up and automating of the technology confirmed that incomplete initial lung filling during TLV was beneficial in human adult-sized pigs, despite larger size and maturity of the lungs. Our results were confirmed in aged non-human primates, confirming the safety of this lung-conservative approach.
INTERPRETATION CONCLUSIONS
This study demonstrated that TLV with an accurate control of perfluorocarbon volume below FRC could provide the full potential of TLV in an innovative and safe manner. This constitutes a new paradigm through the tidal liquid ventilation of incompletely filled lungs, which strongly differs from the previously known TLV approach, opening promising perspectives for a safer clinical translation. FUND: ANR (COOLIVENT), FRM (DBS20140930781), SATT IdfInnov (project 273).

Identifiants

pubmed: 31447395
pii: S2352-3964(19)30548-1
doi: 10.1016/j.ebiom.2019.08.026
pmc: PMC7033528
pii:
doi:

Substances chimiques

Fluorocarbons 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102365

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest R Tissier and A Berdeaux are named as inventor on a patent on cooling with liquid ventilation (US20120226337 A1). P Micheau, M Nadeau and H Walti declares owning patents on liquid ventilation (US Patents # 7,726,311; Preliminary US patent 61/838,896). A Berdeaux, M Kohlhauer, H Walti, M Nadeau, P Micheau and R Tissier are shareholders of a start-up company dedicated to the clinical research on total liquid ventilation (Orixha).

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Auteurs

Matthias Kohlhauer (M)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Emilie Boissady (E)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Fanny Lidouren (F)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Ludovic de Rochefort (L)

Aix Marseille Univ, CNRS, CRMBM UMR 7339, Marseille, France.

Mathieu Nadeau (M)

Université de Sherbrooke, Groupe Inolivent, Sherbrooke, Quebec, Canada.

Jérôme Rambaud (J)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Alice Hutin (A)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Rose-Marie Dubuisson (RM)

IR4M UMR8081 CNRS Univ Paris-Sud, Université Paris Saclay, SHFJ, 4 place du Général Leclerc, 91401, Orsay Cedex, France.

Geneviève Guillot (G)

IR4M UMR8081 CNRS Univ Paris-Sud, Université Paris Saclay, SHFJ, 4 place du Général Leclerc, 91401, Orsay Cedex, France.

Pascaline Pey (P)

Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum - Università di Bologna, Ozzano Emilia, Italy.

Patrick Bruneval (P)

Inserm, UMR 970, Paris Cardiovascular Research Center, Hôpital Européen Georges Pompidou, Paris, France.

Etienne Fortin-Pellerin (E)

Université de Sherbrooke, Groupe Inolivent, Sherbrooke, Quebec, Canada.

Michael Sage (M)

Université de Sherbrooke, Groupe Inolivent, Sherbrooke, Quebec, Canada.

Hervé Walti (H)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Alain Cariou (A)

Service de Médecine Intensive et Réanimation, APHP.Centre, Université de Paris, Hôpital Cochin, Paris, France.

Jean-Damien Ricard (JD)

UMR 1137, Inserm, Université Paris Diderot, Hôpital Louis Mourier, Réanimation médico-chirurgicale, APHP, Colombes, France.

Alain Berdeaux (A)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Nicolas Mongardon (N)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France; Service d'Anesthésie-Réanimation Chirurgicale, Hôpitaux Universitaires Henri Mondor, Créteil, France.

Bijan Ghaleh (B)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Philippe Micheau (P)

Université de Sherbrooke, Groupe Inolivent, Sherbrooke, Quebec, Canada.

Renaud Tissier (R)

U955 - IMRB, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France. Electronic address: renaud.tissier@vet-alfort.fr.

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