Cryptosporidium Diagnostic Assays: Microscopy.
Animals
Coloring Agents
Cryptosporidiosis
/ diagnosis
Cryptosporidium
/ immunology
Feces
/ parasitology
Fluorescent Antibody Technique
/ methods
Humans
Immunoenzyme Techniques
/ methods
Microscopy
/ instrumentation
Microscopy, Fluorescence
/ instrumentation
Oocysts
/ immunology
Sensitivity and Specificity
Staining and Labeling
/ methods
Workflow
Auramine phenol
Cryptosporidium
Immunofluorescent
Microscopy
Oocyst detection
Ziehl–Neelsen
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
28
8
2019
pubmed:
28
8
2019
medline:
24
11
2020
Statut:
ppublish
Résumé
Stained microscopy of fecal smears was the cornerstone of Cryptosporidium diagnosis for many years, and still provides a low-cost method for detecting oocysts. The development and commercialization of improved enzyme immunosorbent assays (EIA) for coproantigen detection provided an automatable method for mass testing, and rapid diagnostics when incorporated onto a cartridge format. Similarly, immunochromatographic lateral flow assays (ICLF) enable rapid diagnostics. Nevertheless, it is important that positive reactions by EIA or ICLF are confirmed. Here we describe microscopical methods using tinctorial stains for the diagnosis of acute cryptosporidiosis, and using immunofluorescent reagents for diagnosis or for confirmation of EIA or ICLF positive reactions.
Identifiants
pubmed: 31452153
doi: 10.1007/978-1-4939-9748-0_1
doi:
Substances chimiques
Coloring Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM