Cryptosporidium Diagnostic Assays: Molecular Detection.
C. hominis
C. parvum
Cryptosporidium
DNA extraction
Detection
Nested PCR
Real-time PCR
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
28
8
2019
pubmed:
28
8
2019
medline:
24
11
2020
Statut:
ppublish
Résumé
Molecular diagnostic assays for Cryptosporidium are usually based on PCR and may detect the entire genus or target specified species. Of the ~40 species, fewer than half have been reported from humans, and most human cases of cryptosporidiosis are caused by Cryptosporidium parvum or Cryptosporidium hominis. Here we describe a nested PCR for the detection of all Cryptosporidium spp. that can then be differentiated by sequencing the PCR amplicons, and a duplex, real-time PCR for the simultaneous detection and differentiation of C. parvum and C. hominis.
Identifiants
pubmed: 31452154
doi: 10.1007/978-1-4939-9748-0_2
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM