Novel methodology to image stromal tissue and assess its morphological features with polarized light: towards a tumour microenvironment prognostic signature.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Aug 2019
01 Aug 2019
Historique:
received:
12
04
2019
revised:
26
05
2019
accepted:
24
06
2019
entrez:
28
8
2019
pubmed:
28
8
2019
medline:
28
8
2019
Statut:
epublish
Résumé
The amount and organization details of peri-tumoural stroma have been linked to patient outcomes in various cancers. In this study, we propose a novel and relatively simple methodology using polarized light microscopy (PLM) to image fibrillar structures within a tumour microenvironment, using only linear crossed polarizers. We demonstrate the technique's ability to image and extract measurement-geometry-independent quantitative morphological metrics related to stromal density and alignment in human invasive breast cancer samples. The findings are promising towards quantitative characterization of peri-tumoural stroma, with potential to develop a PLM signature of tumour microenvironment for providing clinically important information such as breast cancer behaviour or treatment outcome prognosis.
Identifiants
pubmed: 31452988
doi: 10.1364/BOE.10.003963
pii: 364523
pmc: PMC6701544
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3963-3973Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest related to this article.
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