Optimization of enzymatic hydrolysis of cellulosic fraction obtained from stranded driftwood feedstocks for lipid production by
A600, absorbance at 600 nm
ANOVA, analysis of variance
C/N, carbon/nitrogen
C10:0, capric acid (decanoic acid)
C12:0, lauric acid (dodecanoic acid)
C14:0, myristic acid (tetradecanoic acid)
C16:0, palmitic acid (hexadecanoic acid)
C18:0, stearic acid (octadecanoic acid)
C20:0, arachic acid (eicosanoic acid)
C22:0, behenic acid (docosanoic acid)
C24:0, lignoceric acid (tetracosanoic acid)
C5, carbohydrates with five carbon atoms
C6, carbohydrates with six carbon atoms
C8:0, caprylic acid (octanoic acid)
CBU, cellobiase unit
CCD, Central Composite Design
DW, dry weight
ED, enzyme dosage
Enzymatic hydrolysis
Eq, equation
F.A.M.E., fatty acid methyl ester
FA, fatty acid
FPU, filterpaper unit
GC, Gas Chromatography
GC-FID, Gas Chromatography – Flame Ionization Detector
HLF, hydrolyzed liquid fraction
HPLC, high performance liquid chromatography
LF, liquid fraction
NREL, National Renewable Energy Laboratory
PL, total lipid production
PL/DW, % of total intracellular lipid on cellbiomass
PL/d, lipid production per day
RI, refractive index
RSM, response surface methodology
Response surface methodology
Rpm, revolutions per minute
SD, stranded driftwood
SE, steam explosion
SFA, saturated fatty acid
SL, solid loading
Solicoccozyma terricola
Stranded driftwood feedstocks
TAGs, Tryacylglicerols
UFA, unsaturated fatty acid
UI, unsaturation index
WIS, water insoluble substrate
XG, Xilose and Galactose
YL, lipid yied
YPD, Yeast Extract Peptone Dextrose
Yeast biochemicals and biofuels
Yoleic, oleic acid yield
g, gravity force
h, hours
min, minutes
p, p-value
v/v, concentration in volume/volume percent
Δ13C22:1, erucic acid [(13Z)-docos-13-enoic acid]
Δ9,12,15C18:3, linolenic acid [(9Z,12Z,15Z)-9,12,15-octadecatrienoic acid]
Δ9,12C18:2, linoleic acid [(9Z,12Z)-9,12-octadecadienoic acid]
Δ9C16:1, palmitoleic acid [(9Z)-hexadec-9-enoic acid]
Δ9C18:1, oleic acid [(9E9Z)-octadec-9-enoic acid]
Journal
Biotechnology reports (Amsterdam, Netherlands)
ISSN: 2215-017X
Titre abrégé: Biotechnol Rep (Amst)
Pays: Netherlands
ID NLM: 101637426
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
15
03
2019
revised:
04
07
2019
accepted:
06
08
2019
entrez:
28
8
2019
pubmed:
28
8
2019
medline:
28
8
2019
Statut:
epublish
Résumé
Stranded driftwood feedstocks may represent, after pretreatment with steam explosion and enzymatic hydrolysis, a cheap C-source for producing biochemicals and biofuels using oleaginous yeasts. The hydrolysis was optimized using a response surface methodology (RSM). The solid loading (SL) and the dosage of enzyme cocktail (ED) were variated following a central composite design (CCD) aimed at optimizing the conversion of carbohydrates into lipids (Y
Identifiants
pubmed: 31453116
doi: 10.1016/j.btre.2019.e00367
pii: S2215-017X(19)30138-9
pii: e00367
pmc: PMC6704348
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00367Déclaration de conflit d'intérêts
The authors declare that they have no competing interests. On behalf of all co-authors, I confirm that: - There is no conflict of interest. - All authors have approved the manuscript for submission. - The content of the manuscript is original, it has not been published, or submitted for publication elsewhere.
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