Levan Exerts Health Benefit Effect through Alteration in Bifidobacteria Population
Cholesterol
Levan
Journal
Iranian biomedical journal
ISSN: 2008-823X
Titre abrégé: Iran Biomed J
Pays: Iran
ID NLM: 9814853
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
entrez:
29
8
2019
pubmed:
29
8
2019
medline:
21
3
2020
Statut:
ppublish
Résumé
Recently, exploring novel dietary nondigestible carbohydrates, which are able to influence the gut flora, has drawn much attention. The objective of this study was to find out the effective dose of levan, as a prebiotic, in rats in order to further apply in food industry. Levan at various doses (2-10%) was orally administered to male Wistar Albino rats once a day for 90 days. At the end of experiment, fecal and blood samples were collected to measure gut bacteria population and to carry out serum biochemical assay. The rats were sacrificed, and the colon tissues were stained with hematoxylin and eosin and analyzed by histopathology. Of note, levan effectively controlled body weight gain in the rats. Serum biochemical analysis revealed that 5% levan significantly diminished the serum level of total cholesterol, LDL, and glucose as well. More notably, 5% levan intake significantly increased the abundance of bifidobacteria population, highlighting its bifidogenic
effect. Furthermore, our histopathological result revealed that daily intake of levan was associated with a higher degree of thickness of the mucosa layer compared to the rats in control group. Moreover, these findings manifested no colon inflammation in the rats fed with levan. The findings of this study provide the fundamental data to use levan at a definite dose for further development in functional foods.
Sections du résumé
BAckground
Recently, exploring novel dietary nondigestible carbohydrates, which are able to influence the gut flora, has drawn much attention. The objective of this study was to find out the effective dose of levan, as a prebiotic, in rats in order to further apply in food industry.
Methods
Levan at various doses (2-10%) was orally administered to male Wistar Albino rats once a day for 90 days. At the end of experiment, fecal and blood samples were collected to measure gut bacteria population and to carry out serum biochemical assay. The rats were sacrificed, and the colon tissues were stained with hematoxylin and eosin and analyzed by histopathology.
Results
Of note, levan effectively controlled body weight gain in the rats. Serum biochemical analysis revealed that 5% levan significantly diminished the serum level of total cholesterol, LDL, and glucose as well. More notably, 5% levan intake significantly increased the abundance of bifidobacteria population, highlighting its bifidogenic
effect. Furthermore, our histopathological result revealed that daily intake of levan was associated with a higher degree of thickness of the mucosa layer compared to the rats in control group. Moreover, these findings manifested no colon inflammation in the rats fed with levan.
Conclusion
The findings of this study provide the fundamental data to use levan at a definite dose for further development in functional foods.
Substances chimiques
Fructans
0
levan
9013-95-0
Types de publication
Journal Article
Langues
eng
Pagination
54-9Références
Food Funct. 2017 Dec 13;8(12):4374-4383
pubmed: 29068034
Microbiol Res. 2008;163(6):663-70
pubmed: 19216105
FEMS Microbiol Lett. 2000 Jan 1;182(1):163-9
pubmed: 10612749
J Nutr. 2007 Mar;137(3 Suppl 2):830S-7S
pubmed: 17311983
J Agric Food Chem. 2013 Aug 7;61(31):7560-7
pubmed: 23855738
J Food Sci. 2016 Aug;81(8):H2093-8
pubmed: 27434756
Carbohydr Polym. 2015 Apr 20;120:102-14
pubmed: 25662693
Int J Biol Macromol. 2012 May 1;50(4):1070-4
pubmed: 22433476
Plant Physiol. 1999 Jun;120(2):351-60
pubmed: 10364386
Environ Microbiol. 2007 May;9(5):1101-11
pubmed: 17472627
Appl Environ Microbiol. 2006 Jun;72(6):4214-24
pubmed: 16751534
J Appl Microbiol. 2004;97(6):1166-77
pubmed: 15546407
Int J Biol Macromol. 2013 Jul;58:281-6
pubmed: 23624165
J Anim Sci. 2013 Nov;91(11):5287-93
pubmed: 24045474
Syst Appl Microbiol. 2001 Jul;24(2):232-7
pubmed: 11518326
Cell. 2016 Nov 17;167(5):1339-1353.e21
pubmed: 27863247
Biotechnol Adv. 2016 Sep-Oct;34(5):827-844
pubmed: 27178733
Obes Rev. 2011 Sep;12(9):724-39
pubmed: 21676152
Science. 2005 Jun 10;308(5728):1635-8
pubmed: 15831718
J Nutr Biochem. 1999 Jan;10(1):13-8
pubmed: 15539245
Adv Food Nutr Res. 2015;74:47-91
pubmed: 25624035