Highly Sensitive Detection of Uracil-DNA Glycosylase Activity Based on Self-Initiating Multiple Rolling Circle Amplification.


Journal

ACS omega
ISSN: 2470-1343
Titre abrégé: ACS Omega
Pays: United States
ID NLM: 101691658

Informations de publication

Date de publication:
28 Feb 2019
Historique:
received: 02 12 2018
accepted: 08 02 2019
entrez: 29 8 2019
pubmed: 29 8 2019
medline: 29 8 2019
Statut: epublish

Résumé

Sensitive detection of uracil-DNA glycosylase (UDG) activity is very important in the study of many fundamental biochemical processes and clinical applications. Here, we develop a novel assay for the detection of UDG activity by using the self-initiating multiple rolling circle amplification (SM-RCA) strategy. We first design a trigger probe modified with NH

Identifiants

pubmed: 31459598
doi: 10.1021/acsomega.8b03376
pmc: PMC6648713
doi:

Types de publication

Journal Article

Langues

eng

Pagination

3881-3886

Déclaration de conflit d'intérêts

The authors declare no competing financial interest.

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Auteurs

Lijuan Dong (L)

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, and College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.

Xuange Zhang (X)

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, and College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.

Yaru Li (Y)

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, and College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.

Fangjie E (F)

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, and College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.

Jiangyan Zhang (J)

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, and College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.

Yongqiang Cheng (Y)

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, and College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.

Classifications MeSH