Modelling the variable incorporation of aromatic amino acids in the tyrocidines and analogous cyclodecapeptides.
antibiotic
computational modelling
manipulation
peptide production
tyrocidine
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
12
03
2019
revised:
02
08
2019
accepted:
18
08
2019
pubmed:
29
8
2019
medline:
23
1
2020
entrez:
29
8
2019
Statut:
ppublish
Résumé
A mathematical model of the nonribosomal synthesis of tyrocidines and analogues by Brevibacillus parabrevis was constructed using a competitive binding mechanism (CBM) for the incorporation of the three variable aromatic amino acid (Aaa) residues in their sequence. These antimicrobial peptides have a conserved structure (D-Phe Ultra-performance liquid chromatography linked mass spectrometry was used to profile peptides from extracts of cultures grown in media with various Phe : Trp ratios. The CBM model describes the production of peptides as a function of growth medium Aaa concentration. The model accounts for variable Aaa incorporation by simultaneously considering the influence of maximal incorporation rate and cooperativity, despite similar K Our CBM model can be utilized to predict the Aaa composition of produced peptides from the concentration of Aaas in the growth medium. Subtly exploiting the inherent promiscuity of the nontemplate coded peptide synthesis allows for external control of peptide identity, without using genetic manipulation. Such versatility is exploitable in the production of targeted peptide complexes and rare peptides where production processes are reliant on nonribosomal synthesis.
Substances chimiques
Amino Acids, Aromatic
0
Anti-Bacterial Agents
0
Peptides, Cyclic
0
Tyrocidine
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1665-1676Subventions
Organisme : DST/NRF, SARCHI initiative
ID : NRF-SARCHI-82813
Organisme : BIOPEP Peptide Fund, Department of Biochemistry, Stellenbosch University
Informations de copyright
© 2019 The Society for Applied Microbiology.
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