The Pneumatron: An automated pneumatic apparatus for estimating xylem vulnerability to embolism at high temporal resolution.
Citrus sinensis
/ physiology
Databases, Factual
Droughts
Equipment Design
Eucalyptus
Plant Leaves
/ physiology
Plant Roots
/ physiology
Plant Stems
/ physiology
Plant Transpiration
/ physiology
Reproducibility of Results
Sensitivity and Specificity
Trees
/ physiology
Water
/ physiology
Xylem
/ chemistry
cavitation
drought resistance
plant hydraulics
pneumatic method
vulnerability curves
water transport
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
04
06
2019
revised:
16
08
2019
accepted:
20
08
2019
pubmed:
29
8
2019
medline:
24
11
2020
entrez:
29
8
2019
Statut:
ppublish
Résumé
Xylem vulnerability to embolism represents an important trait to determine species distribution patterns and drought resistance. However, estimating embolism resistance frequently requires time-consuming and ambiguous hydraulic lab measurements. Based on a recently developed pneumatic method, we present and test the "Pneumatron", a device that generates high time-resolution and fully automated vulnerability curves. Embolism resistance is estimated by applying a partial vacuum to extract air from an excised xylem sample, while monitoring the pressure change over time. Although the amount of gas extracted is strongly correlated with the percentage loss of xylem conductivity, validation of the Pneumatron was performed by comparison with the optical method for Eucalyptus camaldulensis leaves. The Pneumatron improved the precision of the pneumatic method considerably, facilitating the detection of small differences in the (percentage of air discharged [PAD] < 0.47%). Hence, the Pneumatron can directly measure the 50% PAD without any fitting of vulnerability curves. PAD and embolism frequency based on the optical method were strongly correlated (r
Substances chimiques
Water
059QF0KO0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
131-142Informations de copyright
© 2019 John Wiley & Sons Ltd.
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