Bacteria Detected in both Urine and Open Wounds in Nursing Home Residents: a Pilot Study.
bacteriology
clinical microbiology
nursing home residents
urine microbiota
wound microbiota
Journal
mSphere
ISSN: 2379-5042
Titre abrégé: mSphere
Pays: United States
ID NLM: 101674533
Informations de publication
Date de publication:
28 08 2019
28 08 2019
Historique:
entrez:
30
8
2019
pubmed:
30
8
2019
medline:
23
2
2020
Statut:
epublish
Résumé
Nursing home residents are at a greater risk of developing pressure injuries that develop into an open wound, which can become colonized with bacteria. Understanding the factors that influence microbial colonization of open wounds can lead to the prevention of infections. The relationship between bacteria found in urine and those in open wounds is currently unknown. To determine if bacterial species colonizing open wounds are also found in the urine, we conducted a pilot study with nursing home residents, comparing bacterial species present in the urine with those present in wounds between the umbilicus and mid-thigh. To identify microbial species that were present in both urine and open wound at one time point in one patient, standard clinical bacteriologic culture techniques followed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) were used, as well as 16S rRNA-encoding gene amplicon sequencing. We found some bacterial species detected in urine were also detected in open wounds in one individual at one time point, using both culture-dependent and -independent techniques. Bacterial species that were more often detected, using culture-dependent and -independent methods, at both sites included
Identifiants
pubmed: 31462413
pii: 4/4/e00463-19
doi: 10.1128/mSphere.00463-19
pmc: PMC6714893
pii:
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR000433
Pays : United States
Organisme : AHRQ HHS
ID : K08 HS019767
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM099514
Pays : United States
Organisme : NIA NIH HHS
ID : P30 AG024824
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI124255
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM116149
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002240
Pays : United States
Informations de copyright
Copyright © 2019 Libertucci et al.
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